=====FASTQ format===== @SEQ_ID GATTTGGGGTTCAAAGCAGTATCGATCAAATAGTAAATCCATTTGTTCAACTCACAGTTT + AAAAAAAAAAAAAAEEEEEEEEEEEEEEEEEEEEAAAAAAAAAAAAAAEEEEEAAEEEEE *Line 1 (the header) begins with a '@' character and is followed by a sequence identifier and an optional description. *Line 2 is the raw sequence letters (the base calls; A, C, T, G and N). *Line 3 is a separator, which is simply a plus (+) sign. *Line 4 encodes the quality values for the sequence in Line 2, and must contain the same number of symbols as letters in the sequence.