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general:bioseqanalysis:sequencequalities [2021/10/20 12:33] – ↷ Page moved from general:molmet:sequencequalities to general:bioseqanalysis:sequencequalities ingogeneral:bioseqanalysis:sequencequalities [2021/10/20 12:54] (current) – ingo
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 ===== Storing base quality information ===== ===== Storing base quality information =====
 +==== FASTA ====
 Base quality values are stored differently, depending on the sequence file format. Base quality values are stored differently, depending on the sequence file format.
  
 The [[wp>FASTA|]] format cannot store sequence and base quality information within one file. In this case, each FASTA sequence file is accompanied by a corresponding base quality file. The headers of the corresponding sequence and base quality string must then be identical. Moreover, there must be a one-to-one relationship between nucleotides in the sequence and base quality values in the quality string. The [[wp>FASTA|]] format cannot store sequence and base quality information within one file. In this case, each FASTA sequence file is accompanied by a corresponding base quality file. The headers of the corresponding sequence and base quality string must then be identical. Moreover, there must be a one-to-one relationship between nucleotides in the sequence and base quality values in the quality string.
 +==== FASTQ ====
 The [[wp>FASTQ|]] format stores sequence and quality information within one file. See figure {{ref>FQ}} for an example. The [[wp>FASTQ|]] format stores sequence and quality information within one file. See figure {{ref>FQ}} for an example.