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compgenomics:modul3 [2025/05/17 08:27] – felixcompgenomics:modul3 [2025/09/23 13:26] (current) – felix
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-==== Analysis ====+===== Analysis =====
  
 In this exercise, we will extract the sequences of a subset of orthologs for two PCD gene families from [[https://ebersberger-46-155.biologie.uni-frankfurt.de/phyloppcd/|our interactive webtool]]. We will align these sequences and calculate a phylogenetic tree. This tree will then help us to better understand the evolutionary history of the gene family In this exercise, we will extract the sequences of a subset of orthologs for two PCD gene families from [[https://ebersberger-46-155.biologie.uni-frankfurt.de/phyloppcd/|our interactive webtool]]. We will align these sequences and calculate a phylogenetic tree. This tree will then help us to better understand the evolutionary history of the gene family
  
-=== Task 1: GH27_QRW17738.1 ===+==== Task 1: GH27_QRW17738.1 ====
  
-  - Download the data from the interactive webviewer using this link: [[https://applbio.biologie.uni-frankfurt.de/download/cellulase_pp_data.tar.gz|https://applbio.biologie.uni-frankfurt.de/download/cellulase_pp_data.tar.gz]] +  - Extract the sequences of GH27_QRW17738.1 orthologs for the following taxa:<WRAP>
-  - Unpack the data using ''tar -zxvf cellulase_pp_data.tar.gz'' +
-  - You will find the sequences in the file ''cell_wall.extended.fa'' in the extracted ''data'' directory +
-  - Extract the sequences of GH27_QRW17738.1 orthologs for the following taxa. You find their five letter codes in the header of the Fasta file:<WRAP>+
 <file txt GH27_QRW17738.1_taxa.txt> <file txt GH27_QRW17738.1_taxa.txt>
-Rhizoctonia solani|RHISO +Rhizoctonia solani 
-Aspergillus oryzae|ASPOR +Aspergillus oryzae 
-Trichomonas vaginalis|TRIVA +Trichomonas vaginalis 
-Cordyceps militaris|CORMI +Cordyceps militaris 
-Bradysia coprophila|BRACO +Bradysia coprophila 
-Hydra vulgaris|HYDVU +Hydra vulgaris 
-Notolabrus celidotus|NOTCE +Notolabrus celidotus 
-Homo sapiens|HUMAN+Homo sapiens
 </file></WRAP> </file></WRAP>
 +  - <wrap tip>To get the sequences</wrap> click on the dot in the columns of the respective species in the GH27_QRW17738.1 row (use the search boxes on the left to find them!). 
 +    * In the bottom left corner, you can click on "Detailed plot", opening up a window with more information that also contains the sequence. 
 +    * If no sequence is shown, look at the barcharts on the top of the page. Click on the highest barchart to select one gene from the available options. This will give you the sequence
 +    * Save the sequences in a [[https://de.wikipedia.org/wiki/FASTA-Format|FASTA file]] (use the species name in the FASTA header!)
   - Align the sequences using [[https://www.ebi.ac.uk/jdispatcher/msa/clustalo|ClustalOmega]]   - Align the sequences using [[https://www.ebi.ac.uk/jdispatcher/msa/clustalo|ClustalOmega]]
   - Inspect the distance tree generated by ClustalOmega. What can you learn?   - Inspect the distance tree generated by ClustalOmega. What can you learn?
  
  
-=== Task 2: GH28_QRW23661.1 ===+=== Shortcut to Sequence file === 
 + 
 +<hidden GH27_QRW17738.fa> 
 +<file txt GH27_QRW17738.fa> 
 +>GH27_QRW17738.1|RHISO@456999@016906535_1|XP_043177975.1|1 
 +MIGIPVATFIFLGCAIGIVDAQQPLYTQCGGMVGRSPHHYECCASAIGCWTQSPVGKTPALGWNSWNAYQGNINEAKIRAAADALVSLGLKDAGYNYVNIDDCWSNITGRDVGTSRIKPDLNKFPNGIKSVADYIHSLGLLVGIYSDAGTLTCAGFPGSLGYENIDAQTFAEWGIDYLKYDNCNVPSNWTDTYGGPDFDWYNSNSGIRYRQMTSALASVASIRPIQYGICNWGNADVWTWGSRVGHSWRMSGDSSASWSYITSIIARNTNYLNYVEFYGHNDMDMMEIGNGALTINEQRTHFAMWVALKSPILLGTDLSKLNATQLAIIKNAELLAFSQDTTVGAPAKPYKSGTTTPPEYYTGKSAKGTHVFIMNVGSAPASKIVTFSEVTGLTCTSCKVHDMWTGKGKCIFSHTTTIVFNCGLL 
 +>GH27_QRW17738.1|ASPOR@510516@000184455_2|XP_001820370.1|1 
 +MQRYISLSVSLSLLSGANALVRPDGVGRLPALGWNTWNAFGCDIDASKVLTAAEETINLGLKDAGYEYINIDDCWSVKSGRDPNTKRIIPDSAKFPDGISGVASKIHDLGLKVGIYSSAGTETCAGYPASLGYEKIDAESFAEWGIDYLKYDNCGVPTNWTDTYTHCVPDNSNGSKFPNGTCPDISNPAPTAYDWSSSNTAQRYNAMRDALLGVNRTILYSLCEWGQADVNTWGNGTGNSWRTTGDITPDWSRIVEIANENSFLMNYADFWGYPDPDMLEVGNGNLTLEENRAHFALWAAMKSPLIIGTALDSINEEHLAILKNKPLLSFHQDPVIGRPAYPYKWGYNPDWTFDPAHPAEYWSGPSSTLGGTLVLMFNSEDSAKHRTAVWSEIPELKDSAEKGSGYRVTEIWTGEDLGCVKDQYDVELQSHDIAALVVGESC 
 +>GH27_QRW17738.1|TRIVA@412133@000002825_2|XP_001315888.1|1 
 +MLISFLQLATSTEYETPTMGWSSWNTYRVHISEELIKKQATAMAEKGLKEKGYKYINIDDGYFGGRNETTGQLLINPERFPNGLEPVVTHIHNLGFKAGIYSDGGRNTCGNYYDADVLGKGVGLYAHDDQDADLFFNTLQFDFIKLDYCGGDPNQNSERLNLDEQTRYTSIRKALNKITRSIRVNVCRWAYPGSWVTKLATSWRISSDIENNFDAIKRLINVNMFYSAYAVEGHYNDMDMLEVGRGMSDDEDKTHFGMWCIMSSPLLIGCDMTTIKDSTLELLKNEELIALNQDPLALQAQLVETQSNTFIFVKDILEYHSTTRAFAVYNPSDSEQTVNINFESLGLSGNIKVRDVYAKSDVGTYPSSQPFSVKVNKHGTRIFTISGETRLEQVRYEAENSWIERGSAITSAQVARVDSDSLCSLGHKVTYIGGNENDNYIEWRDVYSQNGGKYNMKIAFLSGEDRILRVIVNGKTNVLKLNAGSWTAVGTKTIEIELQKGSNVIRMDNPISFAPDIDYFEITPV 
 +>GH27_QRW17738.1|CORMI@983644@000225605_1|XP_006673409.1|1 
 +MGFLAALSSASPYSSILDLASTMKSQLVLASAAGLATSTHVDLPLPPMGFNNWARFTTHINQSIFTDAATSMAANGMLAAGYNRLNLDDAWSTHERAANGSMVVDSVKFPQGLSWLAAFIKDKGFIPGIYTDSGTLSCGQYPAAYGHEAIDLQDFSDWGYEYLKLDGCNVPGEDEAAYRRVYFDVWRELLLTFPKPVVFSNSAPAYFCGADNLTDWYSIMGWAQQMGQLARHSYDIINYDSKGSAWDSLMTNYNQHVRLARYQRPNFFNDPDFLNTDHPNYTLEEKKTHMALWSSFSAPLIVSADIPNLKKEELEILLNKDLIAIDQDPLVQQATLVSSSPDWDVLSKNLANGDRVLTILNKAGKAGDYTVPWARIGIQTDRISPDTALSVKDLWTGKTEQVKISAGGITAKNVGPHGTFVYRISGRMGGSSHPIVLPTGLIFNTFSLKCLSDDKSGKVAWKTCDGSDGQTWTVSDEGRVHSLLRPNECLADVQGKLLSRHSGCRTDAWKYYNSGNLVNGNSNRCLTEGAAGAASVSDCGYATNEQVIGLPVGAKVIEVDYYANK 
 +>GH27_QRW17738.1|BRACO@38358@014529535_1|XP_037033872.1|1 
 +MFSASVIIVCVLVTSQVYALENGLARTPPMGWMTWQRFRCTTDCNIFPNDCISENLIKRTVDALVRDGYRDAGYEYVIIDDCWPEMERSSEGLLVPDKNRFPSGMKALSDYIHARGLKFGIYQDYGTKTCAGYPGIIGYQELDAILFASWGVDYVKLDGCYSEPESMDEGYVSFGQLLNKTGRPMVYSCSWPVYQEEKGIEPNFTLISEHCNLWRNWDDINDSWSSLASIADYFAKNQERIQRFAGPGHWNDPDMLLIGNFGLSLEQSKVQMSLWAILAAPLLMSNDLDTIRPEFKDILLNREILSINQDPLGIQGTRVKVIDNIEIWTRPILPRTDSSHSFAVAYVSLRTDGAPHRIQLNLVDIGLTNLGGYVEMDVFEDTVSPTVKYPETLSSVIVNPTSCVLHKFVAL 
 +>GH27_QRW17738.1|BRACO@38358@014529535_1|XP_037037659.1|0 
 +MIYPRSPPHIVLIILSLLAVAYHINGLDNGLARTPPMGWLSWERFRCNTDCEGDPDNCISENLFRTMADLVVSEGYAALGYEYINVDDCWLEKSRGPQGQLVADRKRFPRGMRSLSDYVHSKGLKFGIYEDYGNYTCAGYPGIIGYGRSDAQQFADWNVDYVKLDGCYSLPLDMDQGYPEFGRHLNSTGRQMIYSCSWPVYQIYAGISPNFSSIIEHCNLWRNYDDIQDSWASLETIIDYYGNNQDAIVPNAGPGHWNDPDMLIIGNFGLSYEQSKTQFALWAIMAAPLLMSVDLRTIRPEFKAILQNQKIIAVDQDPLGIQGRRIYKHKGIEIWSRPITPIYQTYYSYAVAFVNRRTDGTPSDVAVTLRELGLQSPTGYRVEDLYEDVNYGILSPNTKIKVKVNPSGVVILSAHVQPERITRTTYNPLNYYHVK 
 +>GH27_QRW17738.1|BRACO@38358@014529535_1|XP_037037657.1|0 
 +MIMIYPRSPPHIVLIILSLLAVAYHINGLDNGLARTPPMGWLSWERFRCNTDCEGDPDNCISENLFRTMADLVVSEGYAALGYEYINVDDCWLEKSRGPQGQLVADRKRFPRGMRSLSDYVHSKGLKFGIYEDYGNYTCAGYPGIIGYGRSDAQQFADWNVDYVKLDGCYSLPLDMDQGYPEFGRHLNSTGRQMIYSCSWPVYQIYAGISPNFSSIIEHCNLWRNYDDIQDSWASLETIIDYYGNNQDAIVPNAGPGHWNDPDMLIIGNFGLSYEQSKTQFALWAIMAAPLLMSVDLRTIRPEFKAILQNQKIIAVDQDPLGIQGRRIYKHKGIEIWSRPITPIYQTYYSYAVAFVNRRTDGTPSDVAVTLRELGLQSPTGYRVEDLYEDVNYGILSPNTKIKVKVNPSGVVILSAHVQPERITRTTYNPLNYYHVK 
 +>GH27_QRW17738.1|BRACO@38358@014529535_1|XP_037050710.1|0 
 +MWSKILSFVLLLWQQNKCLVDALDNGLALTPPMGWMSWQRFRCIIDCDLYPDECISERLFKTAADVIASEGYKDVGYEYVIIDDCWLESERDNVTKELVPDRKRFPNGMKALADYVHSKGLKFGIYENYGAKTCEGYPGIIDHMELDAKTFAKWDVDYIKVDGCYADYLDYDVGYPEFGRLLNKTGRPMVYSCSWPVYQEIKGLTPDYEALKQHCNLWRNWKDIQDSYESLRNITEYFAVNQSRIQPHAGRGHWNDADMLLIGNYGLSIDQSKTQMAIWAVIASPLIMSNDLKNIRPEFKEILLNKEVIDVSQDELGVQGIRKRFEARIETWMRPIKPIVNGELSYAIAWSSKRIDGAPFTVSANVGSLKLTNANGYRVTDLFNKNRFVMDILPAQNITVRVNPSGVVFYKFTAK 
 +>GH27_QRW17738.1|HYDVU@6087@000004095_1|XP_002159485.2|1 
 +MLYLLIISQIVGLYTLDNGLARTPPMGWLQWERFRCIIDCETYPDQCISENLFKRTADKLVSDGYAKLGYKYVNIDDCWPELQRDLDGNLVADKKRFPNGIKALADYIHNHGLKMGIYTDFGTLTCGGYPASIFHMQKDTQIFSNWGVDMVKLDGCYACESLYADGYKAFGWYMNQTERPMLYSCSWPAYVEKNKFDXLLIGDYSLSESQSKVQFALWSILAAPLFISADLDTMKQWQKDILLNEEIIAVNQDVLGKMGRRVYKDNNQQNWVRYLDNNAAAVAIMSLRTDIPVYMEVTFSSINMKGPFHVRDLFLHEDLGVFNTSFKAIVDPVGVVMVKLTPMA 
 +>GH27_QRW17738.1|NOTCE@1203425@009762535_1|XP_034555657.1|1 
 +MHLAVFLLASTLSAITFALDNGEMRTPPMGWLAWERFRCDIDCKNDPKNCISENLFIDMADRLAEDGWRELGYTYVNIDDCWASMERDENGRLQADPKRFPGGIPRLSRYMHDRGLKLGIYGDMGTHTCGGYPGTPLDKIEVDAQTFADWEVDMLKFDGCYSNASEQEKGYPLMSKALNATGRPIGYSCSWPAYQGGLPPKVNYTQLGEICNLWRNYGDIQDSWGSVLSIADWFFENQDILIPAAGPGRWNDPDMLVVGDFGLSMDQSRSQMALWAIMAAPLFMSNDLRTISSGARNILQNKMIISINQDPMGIQGRRIVKEKSHIEVFWRSLSNGASALVFFSRRTDMPYRYQTSLSKLNYTTGSYKIYDVYTDQTTMLKDTTNFVVSVNPTGVVMWYVSAPAKLHERHFYKSGNLMESVHRGDENTIPRVFL 
 +>GH27_QRW17738.1|HUMAN@9606@000001405_39|NP_000160.1|1 
 +MQLRNPELHLGCALALRFLALVSWDIPGARALDNGLARTPTMGWLHWERFMCNLDCQEEPDSCISEKLFMEMAELMVSEGWKDAGYEYLCIDDCWMAPQRDSEGRLQADPQRFPHGIRQLANYVHSKGLKLGIYADVGNKTCAGFPGSFGYYDIDAQTFADWGVDLLKFDGCYCDSLENLADGYKHMSLALNRTGRSIVYSCEWPLYMWPFQKPNYTEIRQYCNHWRNFADIDDSWKSIKSILDWTSFNQERIVDVAGPGGWNDPDMLVIGNFGLSWNQQVTQMALWAIMAAPLFMSNDLRHISPQAKALLQDKDVIAINQDPLGKQGYQLRQGDNFEVWERPLSGLAWAVAMINRQEIGGPRSYTIAVASLGKGVACNPACFITQLLPVKRKLGFYEWTSRLRSHINPTGTVLLQLENTMQMSLKDLL 
 +>GH27_QRW17738.1|HUMAN@9606@000001405_39|NP_000253.1|0 
 +MLLKTVLLLGHVAQVLMLDNGLLQTPPMGWLAWERFRCNINCDEDPKNCISEQLFMEMADRMAQDGWRDMGYTYLNIDDCWIGGRDASGRLMPDPKRFPHGIPFLADYVHSLGLKLGIYADMGNFTCMGYPGTTLDKVVQDAQTFAEWKVDMLKLDGCFSTPEERAQGYPKMAAALNATGRPIAFSCSWPAYEGGLPPRVNYSLLADICNLWRNYDDIQDSWWSVLSILNWFVEHQDILQPVAGPGHWNDPDMLLIGNFGLSLEQSRAQMALWTVLAAPLLMSTDLRTISAQNMDILQNPLMIKINQDPLGIQGRRIHKEKSLIEVYMRPLSNKASALVFFSCRTDMPYRYHSSLGQLNFTGSVIYEAQDVYSGDIISGLRDETNFTVIINPSGVVMWYLYPIKNLEMSQQ 
 +>GH27_QRW17738.1|HUMAN@9606@000001405_39|NP_001349777.1|0 
 +MLLKTVLLLGHVAQVLMLDNGLLQTPPMGWLAWERFRCNINCDEDPKNCISEQLFMEMADRMAQDGWRDMGYTYLNIDDCWIGGRDASGRLMPDPKRFPHGIPFLADYVHSLGLKLGIYADMGNFTCMGYPGTTLDKVVQDAQTFAEWKVDMLKLDGCFSTPEERAQGYPKMAAALNATGRPIAFSCSWPAYEGGLPPRVNYSLLADICNLWRNYDDIQDSWWSVLSILNWFVEHQDILQPVAGPGHWNDPDMLLIGNFGLSLEQSRAQMALWTVLAAPLLMSTDLRTISAQNMDILQNPLMIKINQDPLGIQGRRIHKEKSLIEVYMRPLSNKASALVFFSCRTDMPYRYHSSLGQLNFTGSVIYEAQDVYSGDIISGLRDETNFTVIINPSGVVMWYLYPIKNLEMSQQ 
 +>GH27_QRW17738.1|HUMAN@9606@000001405_39|NP_001349779.1|0 
 +MLLKTVLLLGHVAQVLMLDNGLLQTPPMGWLAWERFRCNINCDEDPKNCISEQLFMEMADRMAQDGWRDMGYTYLNIDDCWIGGRDASGRLMPDPKRFPHGIPFLADYVHSLGLKLGIYADMGNFTCMGYPGTTLDKVVQDAQTFAEWKVDMLKLDGCFSTPEERAQGYPKMAAALNATGRPIAFSCSWPAYEGGLPPRVNYSLLADICNLWRNYDDIQDSWWSVLSILNWFVEHQDILQPVAGPGHWNDPDMLLIGNFGLSLEQSRAQMALWTVLAAPLLMSTDLRTISAQNMDILQNPLMIKINQDPLGIQGRRIHKEKSLIEVYMRPLSNKASALVFFSCRTDMPYRYHSSLGQLNFTGSVIYEAQDVYSGDIISGLRDETNFTVIINPSGVVMWYLYPIKNLEMSQQ 
 +</file> 
 +</hidden> 
 + 
 + 
 +==== Task 2: GH28_QRW23661.1 ====
  
   - Repeat the analysis using GH28_QRW23661.1 and the following taxon set:<WRAP>   - Repeat the analysis using GH28_QRW23661.1 and the following taxon set:<WRAP>
Line 54: Line 91:
 </file></WRAP> </file></WRAP>
  
-=== Summary and Discussion ===+=== Shortcut to Sequence file === 
 + 
 +<hidden GH28_QRW23661.1.fa> 
 +<file> 
 +>WP_367284634.1 glycoside hydrolase family 28 protein [Arcicella rosea] 
 +MKKRISLLIAISSAMFCNSLVAQKTPTYSWKNLPKIVQPTFAKDTFNVTKFGAKPDGITLNTQAINDAIT 
 +ACSKKGGGVVLVPNGMWLTGPIVLKSNVNLHIRKAATLLFTEDKSQYPLVEGSYEGKSAARNQSPISATN 
 +QENIAITGQGIIDGSGDVWRAVNKAQLTESEWNAKKASGGVLKANGLTWYPSEQFMKASVENRSMLLKDG 
 +VSLQSFADMKDFLRPNLLVITKCKKVLLEGVTFQNSPAWCLHPLMSENLTLRNLTIKNPEYAHNGDGMDI 
 +ESCKNFLVDGCTIDVGDDAICIKSGKDEEGRKRGMPTENGIIRNCIVYNGHGGFVVGSEMSGGARNIFVY 
 +DCTFAGTDKGLRFKSVRGRGGIVENIYAKNIFMKDIAQEAIFFDMYYFVKFATDSPRDERPVVNEGTPIF 
 +RNMKFENIVCKGANKGIFVRGLSEMPIQNIQMSNIVLDTKIGAEFIDASNITLEKVTLISENTKPVISVN 
 +NSDGLTFNTIQYKANAELLFAIAGERSKAIQILQTDSSKAQKQIEFTEGATKEAITVSTGK 
 +>GH28_QRW23661.1|PYROR@242507@000002495_2|XP_003719232.1|1 
 +MKLSSLLAMLGVATTATAFMPADRPRNAQEFRAKHPVARRADSGCRRRFTPRASTHDLDDVSAEFEQAVRDANNGGTVHLPKDQLFVIGKPLDLTFLNDIHVKLEGTIRFTNDTPYWQANAFYHPFQRSLMFWKWGGKDIKIYGEGVLDGNGQRWWNEFSGLEILDPDNPYLRPVLFYAENTTNLHVEGIFMKDSPVWHNFVVTSKDVTYKDVIIEAISNNATSPPKNSDFFNSLNVDGVTVERVWVNIGDDCFSPKSNTSNVHVNTMYCNGTHGQSMGSLGQYEGEVSIVENVLIENVALLNGDNGARLKIWAGESVGSGWIRNVTFRNFYAANLDFVARLDSCYFNIPSETCNRFPSKMSIQDITFENFSGTTSGKNGDAVARLTCSTSPDAVCENVVFKNFNITSPCGGAPVVICDGITGLQHGCVAFDSAEAKAAMDNKCKAPVASIEPPWPVRDWRNSK 
 +>GH28_QRW23661.1|RHISO@456999@016906535_1|XP_043183898.1|1 
 +MLSAILAVALGAAVALASKTGVKTQTCIVPSHGNVNISDTPAVHATFKKCGKGGHIIFSENTNYTLRELTTMTPCIGCTVQLEGTIQMADNITYWLKNETTNTPNITAETFPHLVYYPFQDTVAYLILKDWSHSTLVSKTGKGLIDGLGQLWWDAAVGQQILLPGTLRRPVLFTLDGANNVTVDNVTMRNPANWFNWVTDSSNVVYKNIRLSALSANKNPPANADGWDTYRTSHFELRDSHIVSGDDCFAFKPNSTYITIENVYCQNSHGVSVGSLAQYPGVLDIVEHVKVKNVTFVGNGDSSSNGARIKIWSGPVGSAIVNDIHYEDLRVENVTNPLVVDSCYFSSAYCATGKPVATITNVTVTNITGTSTGKVVSSIICPEGSTCDIKLKNVNIVPKTGVAPVYRCFSVASEDLGVNCTYPTIVNGAFKWPA 
 +>GH28_QRW23661.1|PROLA@2754530@002105105_1|XP_040722516.1|1 
 +MLPQHCAIAAAWLQLLSLGYATAQGLSISSSHLLTSNAAVKPNPTEANVAPKVCSIKANMNGISKDLLQAAQACKSNGHIIIEAGDSLIDNVVVMADLKDVTISIQGTLHLKADPVFWAQNAYKHDLVNFQNSSAAILFRDCEGLKFGGAEVFKTGSTIIGYGAPFWSAYLADNTIMRPNLVTLQRCTNCEISNLRFLDTPKWAMYVTDSDHVNIHDMMIQSIGGSVMAMNTDGIDIRNSTNVEFHNNYIDNTDDCVAIKGMCSNIYVHDIICGSKTAGVAIGSLGNIVGVNEFVKDVVIQDVLISGTPRGGIRIKIWPGNRVKAKELLGGGGLADVHNITASRIHTSDNSQALYIDTCYSLGRTNSNCYGYPSLGTITDVTIDGIYGTSPTGSFGHVLCSNPSKCQNIKINNVIMATAVDTASGIGTTAVLNTVGVPPSILSGAPADIGLFDLSLPNGCYLERAAYATDSTPVPYFAPKPRDRPLGYLDGVPKTPADCLQPGSKWWLAQGQPSDFTLSTTSYKPPVYPKVHLSQIPASRVKVSPAFPKNG 
 +>GH28_QRW23661.1|RHAZE@28612@001687245_1|XP_017491101.1|1 
 +LNPVVVQINDSSHIIVSDLTFINSPWFTVAPYRSEYVTIDHVTVKNPSNAPNTDGIHPEACHHVVISNCFVSTGDDGIVITSETDKNTNAKYSSENITVNHCTVHSGHGGIVIGSAISGDVRDIHAHNLHFEGTLRGVRLKSTRDHGGIVENIYIKDITMKNISDEGITISAFYDVKNFDPHNIPSKTFDVSKTPTFRNIHLTNVTGDSKLGLQIVGLPEKHFDKIELKQVHLVAKKEEIIVNADHVLKENFIYKIDKHLHDN 
 +>GH28_QRW23661.1|BRACO@38358@014529535_1|XP_037032572.1|0 
 +MKNLITIATFIGSVLTSQLLPLPTQFFSSRLGDSAPLDKKFIVENCDYNGRLLAFSIENCTDTEGICNIVTKREYNVNITFEPSVSTSNLIWRVIFNINGQDQVLVERPIYEHVQPGVTYTLFNTFAFGFENEGLSFPATFQIIDASTERAEICHSAVLDVNSWHMVPEILSRIKAPIFPDRDFDITTYGAVPDGETDNTEAFSRAIVHCHLLGGGRVVVPPGVFLSSAITLLSNVNLHLKEGSTILFTQNTTAYPNVFTRLGGLELINFSPFIYAFGAENIALTGSGVLNGNADCEHWWPWKGRNNNLELLCGIIEGFPTEEADVAALTEMAERNVPVEERIFGEGHFMRPVFVQPYNSKNILIEGVTFLRSPNWILNPVLCENVIVRGVTINSTGPNSDGCNPESSKDVLIENVKFITGDDCIAVKSGRNADGRRINVKSENIVIQNCEMENGHGGFTIGSEISGGAQNIFCQNCSMNSPQLEQGLRFKNNAVRGGLIEDIYIRNIHIPELYTGTSASRGMVLSIDFFYEEGPNGNYPPVVRNVDIRNVTALKSNYALYLRGFPTDQITNVRLYDCHFNGVVRGSVIEHVENLGLFNVTVNGDVIEVPAV 
 +>GH28_QRW23661.1|SPOLI@69820@002706865_1|XP_022836218.1|1 
 +MFCNSLVAQKTPTYSWKNLPKIVQPTFAKDTFNVTKFGAKPDGITLNTQAINDAITACSKKGGGVVLVPNGMWLTGPIVLKSNVNLHIRKAATLLFTEDKSQYPLVEGSYEGKSAARNQSPISATNQENIAITGQGIIDGSGDVWRAVNKAQLTESEWNAKKASGGVLKANGLTWYPSEQFMKASVENRSMLLKDGVSLQSFADMKDFLRPNLLVITKCKKVLLEGVTFQNSPAWCLHPLMSENLTLRNLTIKNPEYAHNGDGMDIESCKNFLVDGCTIDVGDDAICIKSGKDEEGRKRGMPTENGIIRNCIVYNGHGGFVVGSEMSGGARNIFVYDCTFAGTDKGLRFKSVRGRGGIVENIYAKNIFMKDIAQEAIFFDMYYFVKFATDSPRDERPVVNEGTPIFRNMKFENIVCKGANKGIFVRGLSEMPIQNIQMSNIVLDTKIGAEFIDASNITLEKVTLISENTKPVISVNNSDGLTFNTIQYKANAELLFAIAGERSKAIQILQTDSSKAQKQIEFTEGATKEAITVSTGK 
 +</file> 
 +</hidden> 
 + 
 + 
 + 
 +==== Summary and Discussion ====
  
   * Why are there plant cell wall degrading enzymes in animals?   * Why are there plant cell wall degrading enzymes in animals?
   * Why is GH28 of //Spodoptera litura// identical to a bacterial sequence? What are the consequences given the assumptions stated above?   * Why is GH28 of //Spodoptera litura// identical to a bacterial sequence? What are the consequences given the assumptions stated above?
 +
 +==== Supplementary Data ===
 +
 +{{ :compgenomics:cellulase_all.extended.fa.gz |all_cellulase_sequences.fa.gz}}
  
 ---- ----
  
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