##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	TA100_2P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4331137	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.320199060893245	37.0	37.0	37.0	37.0	37.0
2	36.373509080871834	37.0	37.0	37.0	37.0	37.0
3	36.42986333611705	37.0	37.0	37.0	37.0	37.0
4	36.447663281027594	37.0	37.0	37.0	37.0	37.0
5	36.48183629379537	37.0	37.0	37.0	37.0	37.0
6	36.48514327761971	37.0	37.0	37.0	37.0	37.0
7	36.475291130250554	37.0	37.0	37.0	37.0	37.0
8	36.45022796554346	37.0	37.0	37.0	37.0	37.0
9	36.46027475002523	37.0	37.0	37.0	37.0	37.0
10-14	36.481754837124754	37.0	37.0	37.0	37.0	37.0
15-19	36.48711546182908	37.0	37.0	37.0	37.0	37.0
20-24	36.48816918051772	37.0	37.0	37.0	37.0	37.0
25-29	36.453686456928054	37.0	37.0	37.0	37.0	37.0
30-34	36.42071843952293	37.0	37.0	37.0	37.0	37.0
35-39	36.40992921720094	37.0	37.0	37.0	37.0	37.0
40-44	36.391840756826674	37.0	37.0	37.0	37.0	37.0
45-49	36.37097394979655	37.0	37.0	37.0	37.0	37.0
50-54	36.34039163025956	37.0	37.0	37.0	37.0	37.0
55-59	36.29712876735222	37.0	37.0	37.0	37.0	37.0
60-64	36.29636640013274	37.0	37.0	37.0	37.0	37.0
65-69	36.25364131861012	37.0	37.0	37.0	37.0	37.0
70-74	36.25087299435067	37.0	37.0	37.0	37.0	37.0
75-79	36.22160081635535	37.0	37.0	37.0	37.0	37.0
80-84	36.17553744965712	37.0	37.0	37.0	37.0	37.0
85-89	36.14470676288654	37.0	37.0	37.0	37.0	37.0
90-94	36.121372457289276	37.0	37.0	37.0	37.0	37.0
95-99	36.094472608562754	37.0	37.0	37.0	37.0	37.0
100-104	36.05162915925361	37.0	37.0	37.0	37.0	37.0
105-109	35.98275847973423	37.0	37.0	37.0	37.0	37.0
110-114	35.98249522625899	37.0	37.0	37.0	37.0	37.0
115-119	35.93726150838447	37.0	37.0	37.0	37.0	37.0
120-124	35.897752079047756	37.0	37.0	37.0	37.0	37.0
125-129	35.87352573236023	37.0	37.0	37.0	37.0	37.0
130-134	35.773573379304125	37.0	37.0	37.0	37.0	37.0
135-139	35.71560542248259	37.0	37.0	37.0	37.0	37.0
140-144	35.64413898555417	37.0	37.0	37.0	37.0	37.0
145-149	35.576002312833424	37.0	37.0	37.0	37.0	37.0
150	36.26405735590971	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	1.0
26	34.0
27	252.0
28	1161.0
29	4348.0
30	11962.0
31	27413.0
32	54518.0
33	104381.0
34	219224.0
35	695647.0
36	3032136.0
37	180060.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.583060565159027	28.70077816302529	21.70248452311993	26.013676748695747
2	28.336947088027927	28.80751636348608	23.307113120642455	19.54842342784354
3	23.056901686554824	25.893754919320262	24.44106478275797	26.60827861136695
4	25.082051202721132	24.6554888473858	23.414706115276427	26.84775383461664
5	25.832777859485855	24.495669381965982	23.727834053736927	25.943718704811232
6	25.82375261308113	23.25350130936895	23.836471801350882	27.086274276199042
7	26.90060369828985	23.81164576414923	23.42671220051455	25.86103833704637
8	25.108210615364975	23.673760492914447	24.874415193977935	26.343613697742647
9	25.79911464356819	23.413690215756276	24.76068062497215	26.02651451570338
10-14	25.765216065013146	24.340305899408104	24.207130683168078	25.687347352410665
15-19	25.820866665044306	23.939886622811503	24.226273394613948	26.012973317530243
20-24	25.93826449363802	23.97499611418236	24.100519799106692	25.986219593072928
25-29	26.016967462998046	23.93282169858138	23.971984628700007	26.07822620972056
30-34	26.120059610415492	23.845528452281098	23.86575908799315	26.168652849310252
35-39	26.114382058728918	23.862769383708645	23.86420549959424	26.158643057968195
40-44	26.187723798676966	23.86074398040897	23.785119283563475	26.166412937350593
45-49	26.20915060748274	23.81688644506473	23.736066990655612	26.23789595679692
50-54	26.179803918252663	23.82180867211362	23.800798791324247	26.19758861830947
55-59	26.203861812884494	23.85009120971571	23.76969153438713	26.17635544301267
60-64	26.21324656728194	23.77396173093601	23.75895465471489	26.253837047067158
65-69	26.18546100864329	23.783983073237472	23.790629902152364	26.23992601596687
70-74	26.229778659086943	23.83078448705786	23.735927947521112	26.203508906334083
75-79	26.23609418484771	23.793549020279734	23.72286531677026	26.247491478102297
80-84	26.174958380411432	23.808390986519466	23.771807153682687	26.244843479386414
85-89	26.231899360000305	23.84358549635385	23.71244839120755	26.212066752438297
90-94	26.219815318072026	23.787801227112656	23.725325445364444	26.267058009450878
95-99	26.18207466376766	23.826917578730843	23.767033319362056	26.223974438139436
100-104	26.230170520804613	23.826668808914555	23.744321463315476	26.198839206965356
105-109	26.232226923409762	23.77070390880886	23.71434355868652	26.28272560909486
110-114	26.181004156917638	23.79032679605599	23.743409957106625	26.285259089919748
115-119	26.224081962709754	23.81609518856555	23.724907446281758	26.234915402442944
120-124	26.240718706632908	23.749908458249706	23.72101223215262	26.288360602964765
125-129	26.209168397696896	23.760331107084596	23.756308907575352	26.27419158764316
130-134	26.2311150229888	23.77546799104976	23.719670240612604	26.273746745348838
135-139	26.249893505881566	23.726903864963077	23.695748088460324	26.327454540695033
140-144	26.216946205591874	23.733160869382846	23.738624730427652	26.31126819459763
145-149	26.257898172145676	23.76372872268453	23.688827919940714	26.28954518522908
150	26.66844269153036	23.008040660947408	23.423147452862437	26.900369194659802
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	49.596737313854774
2	47.885370515871465
3	49.665180297921765
4	51.92980503733777
5	51.77649656429709
6	52.91002688928017
7	52.76164203533622
8	51.45182431310762
9	51.82562915927157
10-14	51.45256341742381
15-19	51.833839982574546
20-24	51.92448408671094
25-29	52.09519367271861
30-34	52.28871245972575
35-39	52.27302511669711
40-44	52.354136736027556
45-49	52.44704656427965
50-54	52.37739253656214
55-59	52.380217255897165
60-64	52.4670836143491
65-69	52.42538702461016
70-74	52.433287565421026
75-79	52.483585662950006
80-84	52.41980185979784
85-89	52.4439661124386
90-94	52.4868733275229
95-99	52.4060491019071
100-104	52.42900972776997
105-109	52.51495253250462
110-114	52.466263246837386
115-119	52.45899736515269
120-124	52.52907930959767
125-129	52.48335998534005
130-134	52.504861768337626
135-139	52.5773480465766
140-144	52.5282144001895
145-149	52.54744335737476
150	53.56881188619016
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	15.0
2	4.0
3	0.5
4	0.5
5	0.5
6	0.0
7	0.0
8	0.5
9	1.5
10	2.0
11	4.5
12	12.5
13	19.0
14	39.0
15	74.5
16	109.0
17	202.0
18	348.5
19	510.5
20	833.0
21	1174.5
22	1477.5
23	1946.5
24	2708.0
25	3715.0
26	4911.5
27	6352.0
28	7696.0
29	9072.0
30	11074.5
31	13370.0
32	15835.0
33	18476.0
34	21504.5
35	24679.0
36	28658.5
37	33570.5
38	38828.0
39	44623.0
40	51256.5
41	59157.0
42	67890.0
43	77699.5
44	88539.5
45	101146.5
46	115627.0
47	132747.5
48	151694.5
49	172345.5
50	192762.0
51	210509.5
52	226168.0
53	237867.0
54	245524.0
55	247977.0
56	242784.0
57	230434.5
58	214071.5
59	194084.0
60	169819.5
61	143853.0
62	118615.0
63	95846.0
64	75612.5
65	57389.5
66	42058.0
67	30221.5
68	21950.0
69	15646.0
70	10509.5
71	6922.5
72	4546.5
73	3008.0
74	2079.0
75	1497.0
76	959.0
77	639.5
78	602.0
79	584.0
80	571.5
81	474.5
82	422.5
83	411.0
84	253.5
85	165.5
86	82.5
87	20.5
88	1.0
89	0.0
90	0.5
91	0.5
92	0.5
93	0.5
94	0.5
95	1.0
96	0.5
97	0.5
98	2.0
99	29.5
100	112.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03354777279037814
2	0.0
3	0.0
4	0.0
5	0.0
6	9.928108946911632E-4
7	0.0
8	0.0
9	0.0
10-14	1.847090036634722E-5
15-19	2.770635054952083E-5
20-24	5.079497600745486E-5
25-29	9.23545018317361E-6
30-34	0.0018886495624590033
35-39	2.3550397967092706E-4
40-44	2.8168123058679514E-4
45-49	3.694180073269444E-5
50-54	1.3861806384664849E-5
55-59	8.79240524991741E-5
60-64	1.2977071923938043E-4
65-69	8.819108799349354E-5
70-74	2.3243449519434707E-5
75-79	2.7934534487277917E-5
80-84	2.0049727988138023E-4
85-89	6.350877086479099E-4
90-94	9.354061372090202E-6
95-99	7.027014467029998E-5
100-104	0.0
105-109	3.29042803204012E-5
110-114	3.296374228513044E-5
115-119	1.1795978826312373E-4
120-124	9.455570472768596E-6
125-129	1.516021073829942E-4
130-134	1.4243807647063515E-5
135-139	2.855700048689686E-4
140-144	0.0
145-149	1.4358878240397968E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	6694.0
55-59	6423.0
60-64	6664.0
65-69	6376.0
70-74	6606.0
75-79	6529.0
80-84	6297.0
85-89	6698.0
90-94	6862.0
95-99	6892.0
100-104	7270.0
105-109	7545.0
110-114	8187.0
115-119	8298.0
120-124	8559.0
125-129	9119.0
130-134	9766.0
135-139	10755.0
140-144	11263.0
145-149	126583.0
150-151	4057751.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	62.11537525749785
#Duplication Level	Relative count
1	100.0
2	58.84827401659085
3	28.51806261707252
4	12.563018464008563
5	5.918116135937918
6	2.962804388546963
7	1.8067968959058067
8	1.1827669253411828
9	0.7867273213807867
10++	1.4888948354294889
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1650190	3.4581969	4.252309	8
AAAAA	1668230	3.4121752	4.8116255	1
>>END_MODULE