##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Moltox98_1P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3985368	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.42436131368546	37.0	37.0	37.0	37.0	37.0
2	36.45427348239861	37.0	37.0	37.0	37.0	37.0
3	36.527988381499526	37.0	37.0	37.0	37.0	37.0
4	36.55161380329244	37.0	37.0	37.0	37.0	37.0
5	36.57725535007056	37.0	37.0	37.0	37.0	37.0
6	36.58667254818125	37.0	37.0	37.0	37.0	37.0
7	36.57878343982287	37.0	37.0	37.0	37.0	37.0
8	36.58212792394579	37.0	37.0	37.0	37.0	37.0
9	36.580528573521946	37.0	37.0	37.0	37.0	37.0
10-14	36.58687523962656	37.0	37.0	37.0	37.0	37.0
15-19	36.57896113984957	37.0	37.0	37.0	37.0	37.0
20-24	36.56152455682888	37.0	37.0	37.0	37.0	37.0
25-29	36.53294255386203	37.0	37.0	37.0	37.0	37.0
30-34	36.518649821045386	37.0	37.0	37.0	37.0	37.0
35-39	36.50647152283051	37.0	37.0	37.0	37.0	37.0
40-44	36.4928882853478	37.0	37.0	37.0	37.0	37.0
45-49	36.47290338056611	37.0	37.0	37.0	37.0	37.0
50-54	36.45732600596803	37.0	37.0	37.0	37.0	37.0
55-59	36.452178788841174	37.0	37.0	37.0	37.0	37.0
60-64	36.445153636464404	37.0	37.0	37.0	37.0	37.0
65-69	36.43282989470712	37.0	37.0	37.0	37.0	37.0
70-74	36.42826289191216	37.0	37.0	37.0	37.0	37.0
75-79	36.416961465053234	37.0	37.0	37.0	37.0	37.0
80-84	36.39701897808564	37.0	37.0	37.0	37.0	37.0
85-89	36.37971877829165	37.0	37.0	37.0	37.0	37.0
90-94	36.38791901091854	37.0	37.0	37.0	37.0	37.0
95-99	36.3620704438447	37.0	37.0	37.0	37.0	37.0
100-104	36.350074883249896	37.0	37.0	37.0	37.0	37.0
105-109	36.33784512294797	37.0	37.0	37.0	37.0	37.0
110-114	36.32011170119681	37.0	37.0	37.0	37.0	37.0
115-119	36.302403035327146	37.0	37.0	37.0	37.0	37.0
120-124	36.266219419283196	37.0	37.0	37.0	37.0	37.0
125-129	36.27058264928179	37.0	37.0	37.0	37.0	37.0
130-134	36.2149434835829	37.0	37.0	37.0	37.0	37.0
135-139	36.20768116631875	37.0	37.0	37.0	37.0	37.0
140-144	36.168158394279004	37.0	37.0	37.0	37.0	37.0
145-149	36.10399543156625	37.0	37.0	37.0	37.0	37.0
150	36.51356795015871	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	9.0
27	122.0
28	781.0
29	3626.0
30	11523.0
31	27387.0
32	51674.0
33	86735.0
34	145101.0
35	320009.0
36	2848478.0
37	489923.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.942406322327074	28.855779441195896	21.23058648536346	25.971227751113574
2	28.740942049453817	28.869293092569677	23.852379227116817	18.53738563085969
3	22.564978975582168	25.83341153610484	25.10527263995327	26.496336848359725
4	24.445170433445544	25.08741978156095	23.830195856442867	26.63721392855064
5	25.491027551540423	24.732251760001024	24.02318280850923	25.753537879949324
6	25.673564572771774	23.362207316571336	24.220046148858675	26.74418196179822
7	26.652693007507683	23.948130004715043	23.595768957348053	25.80340803042922
8	24.894263214839885	23.78084031386813	25.086391018345104	26.23850545294688
9	25.62857432488041	23.426719941546175	24.911099803079665	26.033605930493746
10-14	25.76582492908069	24.346224245188033	24.192148161954428	25.695802663776853
15-19	25.783536175329353	24.038121448257726	24.219986711390266	25.958355665022655
20-24	25.91169498023771	24.025224270381056	24.0884254603339	25.974655289047334
25-29	26.015428736577668	23.956621162795447	23.976137805463186	26.0518122951637
30-34	26.12289755927182	23.86365826192211	23.87137649521951	26.14206768358656
35-39	26.145218763071348	23.842222706776013	23.85379512257301	26.158763407579634
40-44	26.182922129148423	23.86560036613934	23.79854005953779	26.152937445174445
45-49	26.214823825553875	23.804933446547473	23.773528567499916	26.206714160398736
50-54	26.18639611441514	23.78796418355576	23.810852111845744	26.21478759018336
55-59	26.20030960935708	23.84322480576489	23.78253010593911	26.17393547893892
60-64	26.206571118817124	23.821609067748856	23.748832709628324	26.222987103805696
65-69	26.191871028023524	23.82373954987962	23.813095400784245	26.17129402131261
70-74	26.203354198031874	23.857989036169343	23.762967014996544	26.175689750802235
75-79	26.226936551541723	23.79292647553301	23.740481203333143	26.239655769592122
80-84	26.181676999437205	23.823551704689276	23.8129473657971	26.18182393007642
85-89	26.202834532527408	23.871435841467914	23.761456019994856	26.164273606009818
90-94	26.221181445091617	23.81553746881509	23.741566120492404	26.221714965600885
95-99	26.1837244998531	23.835702632778446	23.79893965916322	26.18163320820523
100-104	26.200332621141527	23.850262703849427	23.78943825202235	26.159966422986702
105-109	26.23042699936845	23.76614660454353	23.76511587928697	26.23831051680105
110-114	26.188529149394995	23.82176499538028	23.785652450765298	26.204053404459426
115-119	26.23582359820378	23.822086682007058	23.752539076133495	26.189550643655675
120-124	26.226108280767946	23.785853851242738	23.74988545259193	26.238152415397387
125-129	26.190567857065123	23.80095701678845	23.79792321936111	26.21055190678532
130-134	26.224391903307964	23.83232159132616	23.76157626288818	26.181710242477696
135-139	26.225321372570704	23.787212797658214	23.74772771486599	26.23973811490509
140-144	26.21707258628273	23.775068940942052	23.771813066798153	26.23604540597706
145-149	26.246909805641195	23.80245420587737	23.755625990311035	26.1950099981704
150	26.406182843398195	23.42322377976249	23.55150726523531	26.619086111604002
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	49.91363407344064
2	47.2783276803135
3	49.06131582394189
4	51.08238436199618
5	51.244565431489754
6	52.417746534569986
7	52.45610103793691
8	51.13276866778676
9	51.66218025537416
10-14	51.46162759285754
15-19	51.741891840352004
20-24	51.88635026928504
25-29	52.06724103174136
30-34	52.26496524285837
35-39	52.303982170650976
40-44	52.335859574322875
45-49	52.42153798595262
50-54	52.401183704598495
55-59	52.374245088296
60-64	52.429558222622816
65-69	52.36316504933613
70-74	52.379043948834116
75-79	52.466592321133845
80-84	52.36350092951362
85-89	52.36710813853723
90-94	52.442896410692505
95-99	52.36535770805833
100-104	52.360299044128226
105-109	52.468737516169504
110-114	52.392582553854425
115-119	52.425374241859444
120-124	52.46426069616533
125-129	52.401119763850446
130-134	52.40610214578566
135-139	52.4650594874758
140-144	52.45311799225979
145-149	52.4419198038116
150	53.025268955002204
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	3.5
11	10.0
12	14.0
13	31.0
14	60.0
15	76.5
16	110.5
17	204.5
18	321.0
19	457.0
20	758.5
21	1082.0
22	1399.5
23	1882.5
24	2578.5
25	3400.0
26	4393.5
27	5712.0
28	7045.5
29	8512.0
30	10390.0
31	12584.0
32	14800.5
33	17256.5
34	19973.0
35	22832.5
36	26722.0
37	31283.0
38	35923.5
39	41525.0
40	48068.0
41	55171.5
42	62819.0
43	71713.5
44	82277.0
45	93944.5
46	107360.0
47	123463.0
48	141233.0
49	159841.5
50	177888.5
51	193992.0
52	208410.5
53	219644.0
54	226674.0
55	228279.0
56	223206.0
57	211297.5
58	195598.0
59	177218.5
60	154785.0
61	130690.0
62	107822.5
63	87448.0
64	68560.0
65	51521.0
66	37954.5
67	27689.0
68	19738.5
69	13798.5
70	9386.0
71	6034.5
72	3867.5
73	2558.0
74	1828.0
75	1362.0
76	865.5
77	549.5
78	519.0
79	502.0
80	470.5
81	409.5
82	379.5
83	376.0
84	242.0
85	136.0
86	55.5
87	14.0
88	2.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.06375822759654817
3	5.0183571504563695E-5
4	0.0
5	2.5091785752281848E-5
6	1.0036714300912739E-4
7	0.006298038223822744
8	0.0
9	0.0
10-14	0.0016108926452964945
15-19	0.0
20-24	0.0
25-29	0.001289717787667287
30-34	0.0
35-39	7.276617868161736E-4
40-44	0.0
45-49	0.0
50-54	0.0
55-59	6.538205020305401E-5
60-64	0.0011484509336175716
65-69	0.0010391810083314572
70-74	0.0
75-79	3.2885698267849563E-4
80-84	3.2425966957534344E-4
85-89	3.551663023083222E-5
90-94	3.4043573741937517E-4
95-99	0.0
100-104	9.171811415939661E-5
105-109	2.5512994890869696E-5
110-114	1.5330071775396052E-5
115-119	0.0016529437367121618
120-124	2.050061266080937E-4
125-129	5.133328716759707E-6
130-134	1.1311835635326163E-4
135-139	5.150675809871662E-6
140-144	3.095917403400411E-5
145-149	0.001401001173015373
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	6223.0
55-59	6203.0
60-64	5893.0
65-69	5865.0
70-74	5779.0
75-79	5698.0
80-84	5607.0
85-89	5709.0
90-94	5592.0
95-99	5522.0
100-104	5473.0
105-109	5606.0
110-114	5762.0
115-119	5688.0
120-124	6169.0
125-129	6245.0
130-134	6667.0
135-139	6668.0
140-144	6944.0
145-149	67980.0
150-151	3804075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	60.82578663366134
#Duplication Level	Relative count
1	100.0
2	57.76313319847344
3	26.840941794748804
4	11.518007966465767
5	5.267877625075598
6	2.7715793205564014
7	1.5661821442722779
8	0.9947654897708077
9	0.6204250172050635
10++	1.2033117140414173
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1527465	3.4490263	4.2288146	8
AAAAA	1550840	3.4164102	4.6409817	1
>>END_MODULE