##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Moltox100_2P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3507088	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.251991680847475	37.0	37.0	37.0	37.0	37.0
2	36.353443084405065	37.0	37.0	37.0	37.0	37.0
3	36.41132757432947	37.0	37.0	37.0	37.0	37.0
4	36.416107608363404	37.0	37.0	37.0	37.0	37.0
5	36.4491332980524	37.0	37.0	37.0	37.0	37.0
6	36.43092816604545	37.0	37.0	37.0	37.0	37.0
7	36.42382569242631	37.0	37.0	37.0	37.0	37.0
8	36.409374957229474	37.0	37.0	37.0	37.0	37.0
9	36.42643811618072	37.0	37.0	37.0	37.0	37.0
10-14	36.450437742081185	37.0	37.0	37.0	37.0	37.0
15-19	36.44624765617515	37.0	37.0	37.0	37.0	37.0
20-24	36.44586420414886	37.0	37.0	37.0	37.0	37.0
25-29	36.40605704789843	37.0	37.0	37.0	37.0	37.0
30-34	36.37478152815099	37.0	37.0	37.0	37.0	37.0
35-39	36.361957954861694	37.0	37.0	37.0	37.0	37.0
40-44	36.34430193938675	37.0	37.0	37.0	37.0	37.0
45-49	36.31928317738249	37.0	37.0	37.0	37.0	37.0
50-54	36.2887474736508	37.0	37.0	37.0	37.0	37.0
55-59	36.242339193619124	37.0	37.0	37.0	37.0	37.0
60-64	36.24395993076077	37.0	37.0	37.0	37.0	37.0
65-69	36.19669733494952	37.0	37.0	37.0	37.0	37.0
70-74	36.19527451526307	37.0	37.0	37.0	37.0	37.0
75-79	36.162412685557285	37.0	37.0	37.0	37.0	37.0
80-84	36.11529232276248	37.0	37.0	37.0	37.0	37.0
85-89	36.08490640684015	37.0	37.0	37.0	37.0	37.0
90-94	36.061875728778446	37.0	37.0	37.0	37.0	37.0
95-99	36.03586188245235	37.0	37.0	37.0	37.0	37.0
100-104	35.987113168304106	37.0	37.0	37.0	37.0	37.0
105-109	35.91602662808597	37.0	37.0	37.0	37.0	37.0
110-114	35.915320510335036	37.0	37.0	37.0	37.0	37.0
115-119	35.87012284393886	37.0	37.0	37.0	37.0	37.0
120-124	35.826322217295655	37.0	37.0	37.0	37.0	37.0
125-129	35.802199014703	37.0	37.0	37.0	37.0	37.0
130-134	35.69468213180952	37.0	37.0	37.0	37.0	37.0
135-139	35.639848156893194	37.0	37.0	37.0	37.0	37.0
140-144	35.56455963054962	37.0	37.0	37.0	37.0	37.0
145-149	35.49366313775486	37.0	37.0	37.0	37.0	37.0
150	36.231175234853154	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	26.0
27	250.0
28	1215.0
29	4119.0
30	11385.0
31	25251.0
32	49892.0
33	93718.0
34	196069.0
35	611665.0
36	2393555.0
37	119941.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.085879394906595	29.51836972194056	21.309818720130753	26.0859321630221
2	28.82046301661093	29.438810774066692	23.39827229884166	18.342453910480717
3	22.533024549141626	26.21599458011889	24.747568353003974	26.503412517735512
4	24.526758381882633	25.07601748231011	23.594474960423007	26.80274917538425
5	25.496195133968698	24.762481009886265	23.882748308568246	25.858575547576795
6	25.66897068119885	23.399239307375648	24.01992890355388	26.91186110787162
7	26.70585967617579	23.98112051935965	23.47905156642776	25.833968238036796
8	24.807333035270286	23.74140597555579	25.107325507657634	26.34393548151629
9	25.629382553274965	23.423421368383117	24.90062981025854	26.046566268083378
10-14	25.72393979824625	24.36152143579435	24.214721550122846	25.69981721583655
15-19	25.741366067208038	24.026763410974798	24.26311343438169	25.968757087435478
20-24	25.908062834832762	24.009370056616223	24.09022349205748	25.992343616493528
25-29	26.03046916144367	23.927190856685336	23.957643480187404	26.08469650168359
30-34	26.11603478022045	23.85844788569968	23.84985941656921	26.175657917510662
35-39	26.156951609636103	23.831688507448252	23.82162314934277	26.18973673357288
40-44	26.207010646128847	23.830433635115458	23.783306077972156	26.179249640783542
45-49	26.243044724706227	23.781622476231124	23.701242358970248	26.274090440092408
50-54	26.208436012861224	23.799556169922308	23.74876115460191	26.243246662614556
55-59	26.236619449724706	23.817233724605806	23.738669025741714	26.20747779992777
60-64	26.26682449277521	23.7690448170837	23.66860845915648	26.295522230984613
65-69	26.203500717597095	23.791956947844824	23.720415178555044	26.28412715600304
70-74	26.250240651034083	23.80025082286711	23.686048226495956	26.263460299602855
75-79	26.2825164499781	23.75092362340187	23.667635654732237	26.298924271887795
80-84	26.240314275958674	23.80359874012447	23.67927604831625	26.276810935600604
85-89	26.2609061125511	23.81827877283755	23.689164444692892	26.231650669918448
90-94	26.25590146420409	23.77303459808955	23.670462562797965	26.300601374908396
95-99	26.2416584786762	23.753960168869842	23.70774733227215	26.29663402018181
100-104	26.248805913610507	23.805222648773352	23.691753958165577	26.254217479450563
105-109	26.263080974081632	23.727192032149343	23.662122399788984	26.34760459398004
110-114	26.225058474631847	23.750974577197447	23.710560409213123	26.313406538957583
115-119	26.254670688322513	23.7909108950003	23.672010290659486	26.2824081260177
120-124	26.287811324090875	23.7144252268899	23.658669533199024	26.3390939158202
125-129	26.23711725558067	23.733712937885976	23.720914839304353	26.308254967229004
130-134	26.27594952806901	23.743688405725067	23.663842572769912	26.316519493436015
135-139	26.302710532493006	23.695584989743644	23.622209876062644	26.379494601700703
140-144	26.292602371048794	23.712173604922633	23.639158839142706	26.356065184885868
145-149	26.305171906914055	23.754071142219992	23.627840660588188	26.312916290277766
150	26.76525278653218	22.98460766870067	23.30539988493489	26.944739659832262
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	49.1718115579287
2	47.16291692709164
3	49.036437066877134
4	51.329507557266886
5	51.35477068154549
6	52.58083178907047
7	52.53982791421259
8	51.15126851678657
9	51.67594882135834
10-14	51.423757014082796
15-19	51.71012315464352
20-24	51.9004064513263
25-29	52.11516566312726
30-34	52.29169269773111
35-39	52.34668834320898
40-44	52.38626028691239
45-49	52.51713516479863
50-54	52.45168267547577
55-59	52.44409724965248
60-64	52.56234672375982
65-69	52.48762787360014
70-74	52.51370095063693
75-79	52.58144072186589
80-84	52.51712521155928
85-89	52.492556782469556
90-94	52.55650283911248
95-99	52.538292498858006
100-104	52.50302339306108
105-109	52.610685568061676
110-114	52.53846501358943
115-119	52.53707881434021
120-124	52.62690523991108
125-129	52.545372222809675
130-134	52.59246902150502
135-139	52.682205134193715
140-144	52.64866755593466
145-149	52.618088197191824
150	53.70999244636444
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	15.0
2	5.5
3	1.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.5
10	2.0
11	4.5
12	15.5
13	36.5
14	54.0
15	65.5
16	93.0
17	183.5
18	320.0
19	458.5
20	694.0
21	974.5
22	1276.5
23	1676.0
24	2293.0
25	3128.0
26	4160.5
27	5330.5
28	6404.5
29	7573.5
30	9203.0
31	11188.5
32	13019.0
33	15080.5
34	17514.0
35	19997.5
36	23303.5
37	27121.0
38	31180.0
39	35699.0
40	41036.5
41	47124.5
42	54096.0
43	61999.0
44	70807.0
45	81189.5
46	93116.5
47	107118.0
48	122501.0
49	138624.0
50	155198.5
51	170246.5
52	183044.5
53	192625.5
54	198852.0
55	201479.5
56	198315.5
57	188566.0
58	174763.0
59	157864.0
60	138143.5
61	118071.5
62	97800.5
63	78698.5
64	61820.5
65	46642.0
66	34116.5
67	24681.0
68	17519.0
69	12114.5
70	8161.5
71	5351.0
72	3369.5
73	2143.0
74	1433.0
75	939.5
76	606.0
77	405.0
78	339.5
79	305.0
80	274.0
81	228.5
82	190.0
83	173.5
84	122.5
85	73.5
86	28.0
87	6.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.5
97	1.5
98	0.5
99	12.5
100	56.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03373168851195065
2	0.0
3	0.0
4	0.0
5	0.0
6	5.98787370034627E-4
7	0.0
8	0.0
9	0.0
10-14	2.2810947429890553E-5
15-19	3.991915800230847E-5
20-24	3.991915800230847E-5
25-29	2.851368428736319E-5
30-34	0.0017165237940992642
35-39	2.737313691586866E-4
40-44	2.965423165885772E-4
45-49	5.132463171725374E-5
50-54	1.7119988751026058E-5
55-59	1.3719078768433728E-4
60-64	1.3169335785654518E-4
65-69	1.2617580074317548E-4
70-74	3.4471040448031605E-5
75-79	3.453065502062775E-5
80-84	2.3058964134836603E-4
85-89	6.698793154664971E-4
90-94	1.7354692486129694E-5
95-99	1.1011323407494596E-4
100-104	0.0
105-109	5.817576620974644E-5
110-114	3.497546849785784E-5
115-119	9.931254686675925E-5
120-124	1.7564533413099735E-5
125-129	1.6430360912828603E-4
130-134	1.1764523878248236E-5
135-139	3.479717581402832E-4
140-144	5.9145209770315495E-6
145-149	1.7209711190780867E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	6021.0
55-59	5761.0
60-64	5750.0
65-69	5879.0
70-74	6111.0
75-79	5877.0
80-84	5932.0
85-89	6069.0
90-94	6184.0
95-99	6421.0
100-104	6511.0
105-109	6758.0
110-114	7260.0
115-119	7557.0
120-124	7430.0
125-129	8174.0
130-134	8586.0
135-139	9442.0
140-144	9976.0
145-149	109412.0
150-151	3265977.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	56.793595666190306
#Duplication Level	Relative count
1	100.0
2	54.00517292770401
3	23.298366393277117
4	8.90839687316712
5	3.5325904060459794
6	1.343230483736046
7	0.6470967183638934
8	0.29037623914694766
9	0.1288424372373874
10++	0.14807265175043027
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1316010	3.4398868	4.29182	8
AAAAA	1332590	3.3720367	4.9353733	1
>>END_MODULE