##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Moltox100_1P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3507088	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.458814834415335	37.0	37.0	37.0	37.0	37.0
2	36.46900191840068	37.0	37.0	37.0	37.0	37.0
3	36.53710941955263	37.0	37.0	37.0	37.0	37.0
4	36.554218200398736	37.0	37.0	37.0	37.0	37.0
5	36.576243880963354	37.0	37.0	37.0	37.0	37.0
6	36.58282341361266	37.0	37.0	37.0	37.0	37.0
7	36.57836929099013	37.0	37.0	37.0	37.0	37.0
8	36.57828489048464	37.0	37.0	37.0	37.0	37.0
9	36.58055343920655	37.0	37.0	37.0	37.0	37.0
10-14	36.58505255642287	37.0	37.0	37.0	37.0	37.0
15-19	36.57433699981294	37.0	37.0	37.0	37.0	37.0
20-24	36.55739679186835	37.0	37.0	37.0	37.0	37.0
25-29	36.528729932069	37.0	37.0	37.0	37.0	37.0
30-34	36.51585691605115	37.0	37.0	37.0	37.0	37.0
35-39	36.50375998549224	37.0	37.0	37.0	37.0	37.0
40-44	36.488543657872285	37.0	37.0	37.0	37.0	37.0
45-49	36.47185630927994	37.0	37.0	37.0	37.0	37.0
50-54	36.45277137681767	37.0	37.0	37.0	37.0	37.0
55-59	36.450475590186834	37.0	37.0	37.0	37.0	37.0
60-64	36.442918929254176	37.0	37.0	37.0	37.0	37.0
65-69	36.43156868775937	37.0	37.0	37.0	37.0	37.0
70-74	36.42822997725925	37.0	37.0	37.0	37.0	37.0
75-79	36.4163161445106	37.0	37.0	37.0	37.0	37.0
80-84	36.39534861037289	37.0	37.0	37.0	37.0	37.0
85-89	36.37987572938988	37.0	37.0	37.0	37.0	37.0
90-94	36.3899628672532	37.0	37.0	37.0	37.0	37.0
95-99	36.362550576340155	37.0	37.0	37.0	37.0	37.0
100-104	36.350015903328575	37.0	37.0	37.0	37.0	37.0
105-109	36.33869659810915	37.0	37.0	37.0	37.0	37.0
110-114	36.32423208163059	37.0	37.0	37.0	37.0	37.0
115-119	36.30498136121486	37.0	37.0	37.0	37.0	37.0
120-124	36.26859472885899	37.0	37.0	37.0	37.0	37.0
125-129	36.27458589540564	37.0	37.0	37.0	37.0	37.0
130-134	36.21904967205182	37.0	37.0	37.0	37.0	37.0
135-139	36.211915259319255	37.0	37.0	37.0	37.0	37.0
140-144	36.173002447240435	37.0	37.0	37.0	37.0	37.0
145-149	36.11119205126842	37.0	37.0	37.0	37.0	37.0
150	36.51767469572082	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	10.0
27	104.0
28	735.0
29	3345.0
30	10640.0
31	25281.0
32	47091.0
33	77780.0
34	127496.0
35	277232.0
36	2492246.0
37	445128.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.515435027578434	29.341122891698184	21.34850907647598	25.7949330042474
2	28.91728653570551	29.020028206169236	23.658430879906373	18.40425437821888
3	22.594826588227377	25.930844011238964	24.997761674506982	26.47656772602668
4	24.547601885096697	25.015796581095202	23.718965706021635	26.717635827786474
5	25.535287395126673	24.622193683192435	24.001849967836563	25.840668953844332
6	25.6907374643044	23.311411043644508	24.147832174013462	26.85001931803763
7	26.663934907716165	23.913270360301325	23.60188405821897	25.820910673763535
8	24.95166930513292	23.70322615229501	25.070542854926934	26.274561687645136
9	25.715864557718543	23.356385696623523	24.82438421847413	26.103365527183808
10-14	25.79735744821893	24.31554135881872	24.15426549565639	25.732835697305962
15-19	25.79590246951317	24.01935166725215	24.214362456830283	25.9703834064044
20-24	25.948901196662298	23.984182889052114	24.067511280013505	25.99940463427208
25-29	26.069325903556535	23.933611045116894	23.92427554030446	26.07278751102211
30-34	26.150441619942242	23.851029686166985	23.83707508907675	26.16145360481402
35-39	26.170042204511816	23.82775937651355	23.821611787266722	26.180586631707907
40-44	26.22129242265948	23.8477563152108	23.773734790800802	26.15721647132892
45-49	26.254094565063667	23.772828055640463	23.743196634929035	26.229880744366834
50-54	26.22837840189844	23.76688234409609	23.793480372897402	26.21125888110807
55-59	26.258527469518878	23.80682872014296	23.75089486452787	26.183748945810294
60-64	26.26499946273828	23.755119943893504	23.715968820844772	26.263911772523446
65-69	26.23551038051124	23.773522831838395	23.757382880861417	26.233583906788944
70-74	26.278959101710058	23.804117317283126	23.70667658779321	26.210246993213605
75-79	26.24109608641289	23.774569257670873	23.716445310294006	26.26788934562223
80-84	26.227675145437278	23.792254790801326	23.758419109425287	26.221650954336113
85-89	26.238710542594745	23.838362165303295	23.706890112631417	26.21603717947054
90-94	26.26046125555573	23.790921895934606	23.696872042349582	26.251744806160087
95-99	26.221239575884574	23.79758320984375	23.754627460975115	26.226549753296563
100-104	26.257166412782595	23.819960432086436	23.718103641239793	26.204769513891176
105-109	26.256232818832036	23.773222476615164	23.724133218107195	26.246411486445602
110-114	26.227566541492337	23.79058855871975	23.755684289256003	26.226160610531913
115-119	26.257757090909088	23.805230545454545	23.725376	26.211636363636366
120-124	26.243609650633708	23.747527650919924	23.730041156483672	26.278821541962692
125-129	26.212493047648337	23.767570555061752	23.765247754674927	26.254688642614983
130-134	26.276281077483915	23.80303646139898	23.71091973680034	26.209762724316764
135-139	26.26470486618418	23.727418964761025	23.71006211340161	26.297814055653184
140-144	26.246108349555385	23.754748118585876	23.7423586616383	26.25678487022044
145-149	26.271929994699665	23.80000477253297	23.691259199909535	26.236806032857828
150	26.438181143445515	23.33383730304097	23.583750502724723	26.64423105078879
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	49.31036803182584
2	47.321540913924395
3	49.07139431425406
4	51.26523771288317
5	51.375956348970995
6	52.54075678234204
7	52.4848455814797
8	51.226230992778056
9	51.819230084902344
10-14	51.53019314552489
15-19	51.766285875917575
20-24	51.94830583093438
25-29	52.14211341457865
30-34	52.31189522475626
35-39	52.35062883621973
40-44	52.37850889398841
45-49	52.4839753094305
50-54	52.43963728300651
55-59	52.44227641532917
60-64	52.528911235261724
65-69	52.46909428730019
70-74	52.48920609492366
75-79	52.508985432035125
80-84	52.44932609977339
85-89	52.454747722065285
90-94	52.512206061715816
95-99	52.44778932918114
100-104	52.461935926673775
105-109	52.50264430527765
110-114	52.45372715202424
115-119	52.469393454545454
120-124	52.5224311925964
125-129	52.46718169026332
130-134	52.486043801800676
135-139	52.562518921837366
140-144	52.50289321977583
145-149	52.508736027557504
150	53.08241219423431
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.5
8	2.0
9	1.0
10	1.0
11	2.5
12	7.0
13	29.5
14	57.5
15	64.0
16	92.0
17	181.5
18	306.5
19	446.0
20	677.5
21	967.5
22	1267.5
23	1717.5
24	2324.5
25	3045.5
26	4016.5
27	5260.0
28	6471.0
29	7759.0
30	9156.5
31	10888.0
32	13013.0
33	15251.0
34	17815.0
35	20258.5
36	23337.0
37	27245.5
38	31080.0
39	35598.0
40	41361.5
41	47507.0
42	53963.5
43	61579.0
44	70865.0
45	81328.5
46	93048.0
47	107030.0
48	122622.5
49	139142.0
50	155176.5
51	169772.5
52	182485.5
53	192309.0
54	199163.0
55	201679.0
56	198239.0
57	188413.0
58	174466.0
59	158016.5
60	138625.0
61	117878.0
62	97560.0
63	78637.0
64	61218.0
65	46208.5
66	34093.0
67	24542.5
68	17393.0
69	11945.0
70	7967.5
71	5195.0
72	3388.0
73	2196.5
74	1429.5
75	964.0
76	611.0
77	416.5
78	339.5
79	295.0
80	290.5
81	237.5
82	190.0
83	205.0
84	143.5
85	68.5
86	24.5
87	8.0
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.062188345430739124
3	5.702736857472638E-5
4	0.0
5	0.0
6	8.554105286208957E-5
7	0.005417600014599006
8	0.0
9	0.0
10-14	0.001528333477802667
15-19	0.0
20-24	0.0
25-29	0.0013344404246485974
30-34	0.0
35-39	6.330037911794628E-4
40-44	0.0
45-49	0.0
50-54	0.0
55-59	1.2002958900839898E-4
60-64	0.0010361573104333026
65-69	0.0010836297261306472
70-74	0.0
75-79	3.105455042327352E-4
80-84	2.99480878693024E-4
85-89	4.037483535718925E-5
90-94	2.0217038573531971E-4
95-99	5.7845066501291476E-6
100-104	8.689362083227058E-5
105-109	2.900569857556495E-5
110-114	0.0
115-119	0.0017861499142968033
120-124	1.922869732742667E-4
125-129	5.836181535827355E-6
130-134	1.0522269945912609E-4
135-139	2.9279430292557138E-5
140-144	2.3464875076524824E-5
145-149	0.001557514209672328
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	5708.0
55-59	5536.0
60-64	5360.0
65-69	5430.0
70-74	5235.0
75-79	5142.0
80-84	5086.0
85-89	5162.0
90-94	4906.0
95-99	5068.0
100-104	4897.0
105-109	5009.0
110-114	4962.0
115-119	5065.0
120-124	5438.0
125-129	5450.0
130-134	5935.0
135-139	5870.0
140-144	6036.0
145-149	59031.0
150-151	3346762.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	57.214714310002364
#Duplication Level	Relative count
1	100.0
2	54.46606834790362
3	23.718234507356218
4	9.407044137316095
5	3.5947585725639186
6	1.4247223245265463
7	0.6493632363488341
8	0.29172885692686423
9	0.1444106301730989
10++	0.1434414313128768
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1326390	3.4351614	4.1793985	8
AAAAA	1351245	3.4050899	5.034374	1
>>END_MODULE