##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ICCR98_2P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3640159	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.15966170708477	37.0	37.0	37.0	37.0	37.0
2	36.225626682790505	37.0	37.0	37.0	37.0	37.0
3	36.30538693502125	37.0	37.0	37.0	37.0	37.0
4	36.315682639137464	37.0	37.0	37.0	37.0	37.0
5	36.35440457408591	37.0	37.0	37.0	37.0	37.0
6	36.36492471894772	37.0	37.0	37.0	37.0	37.0
7	36.32539595111093	37.0	37.0	37.0	37.0	37.0
8	36.33512739416053	37.0	37.0	37.0	37.0	37.0
9	36.33944259028246	37.0	37.0	37.0	37.0	37.0
10-14	36.358807238914565	37.0	37.0	37.0	37.0	37.0
15-19	36.3589037182167	37.0	37.0	37.0	37.0	37.0
20-24	36.3583428635947	37.0	37.0	37.0	37.0	37.0
25-29	36.3143914867455	37.0	37.0	37.0	37.0	37.0
30-34	36.278175321462605	37.0	37.0	37.0	37.0	37.0
35-39	36.26375617109033	37.0	37.0	37.0	37.0	37.0
40-44	36.241143807179846	37.0	37.0	37.0	37.0	37.0
45-49	36.212980586837006	37.0	37.0	37.0	37.0	37.0
50-54	36.178088170430875	37.0	37.0	37.0	37.0	37.0
55-59	36.1254370610918	37.0	37.0	37.0	37.0	37.0
60-64	36.12806716866085	37.0	37.0	37.0	37.0	37.0
65-69	36.073116018896584	37.0	37.0	37.0	37.0	37.0
70-74	36.07194801922954	37.0	37.0	37.0	37.0	37.0
75-79	36.036180767516655	37.0	37.0	37.0	37.0	37.0
80-84	35.9825221540577	37.0	37.0	37.0	37.0	37.0
85-89	35.94490893104573	37.0	37.0	37.0	37.0	37.0
90-94	35.92087852468609	37.0	37.0	37.0	37.0	37.0
95-99	35.8930779188748	37.0	37.0	37.0	37.0	37.0
100-104	35.8379960250982	37.0	37.0	37.0	37.0	37.0
105-109	35.75834720295313	37.0	37.0	37.0	37.0	37.0
110-114	35.760873212767095	37.0	37.0	37.0	37.0	37.0
115-119	35.707412534877164	37.0	37.0	37.0	37.0	37.0
120-124	35.66391769008808	37.0	37.0	37.0	37.0	37.0
125-129	35.6365079160984	37.0	37.0	37.0	37.0	37.0
130-134	35.519286554159905	37.0	37.0	37.0	37.0	37.0
135-139	35.457797254466115	37.0	37.0	37.0	37.0	37.0
140-144	35.38048916318871	37.0	37.0	37.0	34.6	37.0
145-149	35.306856879825276	37.0	37.0	37.0	27.4	37.0
150	36.15966479162075	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	40.0
27	328.0
28	1457.0
29	5082.0
30	13736.0
31	30941.0
32	61940.0
33	118971.0
34	256752.0
35	779379.0
36	2285076.0
37	86455.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.606632520985634	29.50253344538129	21.19052971240352	25.70030432122955
2	28.327828537160055	29.105898945622982	23.934641316491945	18.631631200725025
3	22.597611807616097	26.05957596907168	24.883748209899622	26.459064013412604
4	24.610078845457025	25.07335531222675	23.717974956588435	26.598590885727795
5	25.405675960857753	24.713563336107022	24.054910788237546	25.825849914797676
6	25.6964605562454	23.35958704658088	24.219503752623538	26.72444864455018
7	26.551807215014513	23.963129083097744	23.660999423376836	25.82406427851091
8	24.86517759251725	23.785993963450498	25.14162156103621	26.207206882996044
9	25.664428394473976	23.459085166334766	24.87204542438943	26.004441014801827
10-14	25.70261117858378	24.367146801635204	24.269090591422295	25.66115142835872
15-19	25.730876523279246	24.07049740662256	24.27390623798987	25.924719832108323
20-24	25.901034037931225	24.030394736314367	24.106254110056692	25.962317115697715
25-29	26.015476358639976	23.957274828629718	23.967637014497114	26.059611798233185
30-34	26.09734993860603	23.89708427534125	23.866568603340436	26.13899718271228
35-39	26.135651377767854	23.86200805857167	23.840596845931135	26.16174371772934
40-44	26.186024383729826	23.88130528517435	23.77319980838145	26.159470522714372
45-49	26.19583283976496	23.803941895262994	23.736598657157042	26.263626607815006
50-54	26.184429581986212	23.807767590783886	23.790160365420864	26.21764246180904
55-59	26.20683476160194	23.856794148752076	23.75207300207288	26.184298087573104
60-64	26.2125582466539	23.79512505785945	23.708264789588167	26.284051905898487
65-69	26.210363072543082	23.795487947700565	23.759529762770594	26.234619216985767
70-74	26.22198004451235	23.86376905028747	23.700620669091325	26.213630236108855
75-79	26.222990109448563	23.804295358214684	23.711593662437593	26.26112086989916
80-84	26.186936177826947	23.800587484624486	23.75379982699033	26.25867651055823
85-89	26.252246897358265	23.843520486119267	23.67991450199538	26.22431811452709
90-94	26.25201805380638	23.784992528832834	23.680628221023543	26.282361196337238
95-99	26.198168270627693	23.79045311863876	23.728620274100955	26.2827583366326
100-104	26.262225078611557	23.82153438210154	23.67715715717065	26.239083382116252
105-109	26.258920965667805	23.762106305934893	23.682073734356162	26.29689899404114
110-114	26.21263871604902	23.792647855353987	23.696445239390236	26.29826818920676
115-119	26.24570773282537	23.825934064640244	23.678869272639762	26.249488929894625
120-124	26.278605534589893	23.728407194407207	23.662190685722294	26.330796585280602
125-129	26.226397104967692	23.764572284021682	23.709506333238938	26.29952427777169
130-134	26.27778645587121	23.758518804743353	23.651041759442336	26.312652979943095
135-139	26.29347513900325	23.703815424723327	23.636394568617074	26.366314867656353
140-144	26.287664409247707	23.686204871621452	23.6738106728653	26.352320046265543
145-149	26.295231583026315	23.710847635951655	23.630432212263003	26.363488568759024
150	26.81397516123159	22.806753279733215	23.360043554090442	27.019228004944758
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	49.30693684221519
2	46.959459737885076
3	49.0566758210287
4	51.20866973118482
5	51.231525875655436
6	52.42090920079557
7	52.375871493525416
8	51.0723844755133
9	51.66886940927581
10-14	51.3637626069425
15-19	51.655596355387566
20-24	51.86335115362895
25-29	52.075088156873164
30-34	52.23634712131832
35-39	52.29739509549719
40-44	52.3454949064442
45-49	52.45945944757996
50-54	52.40207204379526
55-59	52.39113284917505
60-64	52.49661015255238
65-69	52.44498228952884
70-74	52.435610280621205
75-79	52.48411097934772
80-84	52.44561268838518
85-89	52.476565011885356
90-94	52.53437925014362
95-99	52.480926607260294
100-104	52.50130846072781
105-109	52.55581995970894
110-114	52.51090690525578
115-119	52.49519666271999
120-124	52.609402119870495
125-129	52.525921382739384
130-134	52.590439435814304
135-139	52.6597900066596
140-144	52.63998445551324
145-149	52.658720151785346
150	53.83320316617635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	17.0
2	3.5
3	0.5
4	1.5
5	1.5
6	1.0
7	1.0
8	1.0
9	1.0
10	2.5
11	9.5
12	18.0
13	37.0
14	47.5
15	63.5
16	122.0
17	201.5
18	315.5
19	465.0
20	758.0
21	1079.0
22	1344.0
23	1772.5
24	2404.0
25	3208.5
26	4283.0
27	5436.5
28	6523.0
29	7862.0
30	9548.0
31	11537.0
32	13668.0
33	15643.0
34	17996.0
35	20680.0
36	24226.0
37	28445.5
38	33070.0
39	38429.0
40	44265.5
41	50740.5
42	57771.5
43	65940.5
44	75429.5
45	85885.0
46	97546.0
47	112419.0
48	128891.0
49	145885.5
50	162190.0
51	176696.5
52	190132.0
53	199854.0
54	205932.5
55	207944.5
56	203932.0
57	193393.0
58	179120.0
59	162597.5
60	142289.5
61	120288.5
62	99316.5
63	80568.0
64	63274.0
65	47709.5
66	35247.0
67	25577.5
68	18388.5
69	12857.0
70	8648.5
71	5743.0
72	3809.0
73	2567.5
74	1806.5
75	1280.5
76	846.5
77	575.5
78	489.5
79	480.5
80	494.0
81	424.5
82	353.5
83	321.5
84	215.0
85	132.0
86	49.0
87	13.0
88	2.5
89	1.5
90	2.0
91	1.0
92	1.0
93	1.0
94	0.0
95	0.0
96	0.5
97	1.0
98	1.0
99	14.5
100	56.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0340919174134976
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0010713817720599567
7	0.0
8	0.0
9	0.0
10-14	5.494265497743368E-5
15-19	6.593118597292042E-5
20-24	6.593118597292042E-5
25-29	1.0988530995486735E-5
30-34	0.0017087165697981872
35-39	2.8020754038491177E-4
40-44	2.857018058826551E-4
45-49	3.845985848420358E-5
50-54	2.7494098254339213E-5
55-59	9.367312948811382E-5
60-64	1.3803977555395085E-4
65-69	1.7151897536555948E-4
70-74	2.2177441040441342E-5
75-79	5.000388641317178E-5
80-84	2.2269182980420602E-4
85-89	6.527459571872296E-4
90-94	1.1182288239470045E-5
95-99	7.845379231342245E-5
100-104	0.0
105-109	3.941380190262809E-5
110-114	2.822514634738381E-5
115-119	1.2453028732250542E-4
120-124	2.838011738924716E-5
125-129	1.4230378442684304E-4
130-134	1.7127813050605666E-5
135-139	2.806807711618265E-4
140-144	1.7246099191054473E-5
145-149	1.7897003897216927E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	7565.0
55-59	7425.0
60-64	7542.0
65-69	7311.0
70-74	7602.0
75-79	7363.0
80-84	7399.0
85-89	7733.0
90-94	8011.0
95-99	8136.0
100-104	8420.0
105-109	8935.0
110-114	9575.0
115-119	9684.0
120-124	9625.0
125-129	10526.0
130-134	10943.0
135-139	12320.0
140-144	12969.0
145-149	131779.0
150-151	3339296.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	57.378219037701164
#Duplication Level	Relative count
1	100.0
2	52.089944514747394
3	22.908595347026186
4	9.486031344300594
5	4.295726662124014
6	2.259320549011973
7	1.353061423148058
8	0.8644018300399104
9	0.534410590869269
10++	0.8936045945682858
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1369950	3.445196	4.161216	8
AAAAA	1399385	3.410345	4.8886037	1
>>END_MODULE