##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ICCR98_1P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3640159	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.44047718794701	37.0	37.0	37.0	37.0	37.0
2	36.469679209067515	37.0	37.0	37.0	37.0	37.0
3	36.545060531696556	37.0	37.0	37.0	37.0	37.0
4	36.56345698086265	37.0	37.0	37.0	37.0	37.0
5	36.587947669318844	37.0	37.0	37.0	37.0	37.0
6	36.59516631004305	37.0	37.0	37.0	37.0	37.0
7	36.58838583699229	37.0	37.0	37.0	37.0	37.0
8	36.592800754033	37.0	37.0	37.0	37.0	37.0
9	36.5946330915765	37.0	37.0	37.0	37.0	37.0
10-14	36.59712067522325	37.0	37.0	37.0	37.0	37.0
15-19	36.58750823796433	37.0	37.0	37.0	37.0	37.0
20-24	36.569622535718906	37.0	37.0	37.0	37.0	37.0
25-29	36.54251745596827	37.0	37.0	37.0	37.0	37.0
30-34	36.52803083601568	37.0	37.0	37.0	37.0	37.0
35-39	36.517612774606825	37.0	37.0	37.0	37.0	37.0
40-44	36.50208229915232	37.0	37.0	37.0	37.0	37.0
45-49	36.48604036252263	37.0	37.0	37.0	37.0	37.0
50-54	36.4669635350938	37.0	37.0	37.0	37.0	37.0
55-59	36.46361882936973	37.0	37.0	37.0	37.0	37.0
60-64	36.454555016848225	37.0	37.0	37.0	37.0	37.0
65-69	36.44441425784725	37.0	37.0	37.0	37.0	37.0
70-74	36.43900694316216	37.0	37.0	37.0	37.0	37.0
75-79	36.42732405333491	37.0	37.0	37.0	37.0	37.0
80-84	36.405893234159244	37.0	37.0	37.0	37.0	37.0
85-89	36.388451101715184	37.0	37.0	37.0	37.0	37.0
90-94	36.39962997053245	37.0	37.0	37.0	37.0	37.0
95-99	36.37066884828007	37.0	37.0	37.0	37.0	37.0
100-104	36.35993570307137	37.0	37.0	37.0	37.0	37.0
105-109	36.345490347577716	37.0	37.0	37.0	37.0	37.0
110-114	36.33295337233716	37.0	37.0	37.0	37.0	37.0
115-119	36.311168622837556	37.0	37.0	37.0	37.0	37.0
120-124	36.27654967758791	37.0	37.0	37.0	37.0	37.0
125-129	36.28120847145813	37.0	37.0	37.0	37.0	37.0
130-134	36.226052644631785	37.0	37.0	37.0	37.0	37.0
135-139	36.216240368915756	37.0	37.0	37.0	37.0	37.0
140-144	36.17631707833108	37.0	37.0	37.0	37.0	37.0
145-149	36.11425412053963	37.0	37.0	37.0	37.0	37.0
150	36.51725771692606	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	13.0
27	99.0
28	663.0
29	3152.0
30	9822.0
31	23979.0
32	45845.0
33	78310.0
34	130882.0
35	287290.0
36	2598131.0
37	461973.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.952168023429746	29.30080801415543	21.30165193333588	25.44537202907895
2	28.393024871237422	28.721648798742788	24.19601214657809	18.689314183441695
3	22.65346101640066	25.6728895633405	25.200575029826993	26.473074390431844
4	24.574530947686625	25.072805885676974	23.8617049420094	26.490958224627004
5	25.450783880594223	24.67914176276366	24.066064147197967	25.80401020944415
6	25.743814276091463	23.39273371800549	24.183963544419527	26.67948846148352
7	26.58529911449187	23.958830135848245	23.646270382181704	25.80960036747818
8	25.025307960448977	23.801020779586825	24.968854382459668	26.204816877504527
9	25.65808251782408	23.42304278466957	24.82542658164108	26.09344811586527
10-14	25.771432926093894	24.325550603985725	24.195653166479428	25.70736330344095
15-19	25.788648243112455	24.037807139743073	24.24099057211512	25.93255404502935
20-24	25.9387680593073	23.995633157782393	24.092727817658513	25.972870965251794
25-29	26.018022191733543	23.98849683809197	23.968305152791718	26.025175817382774
30-34	26.108337575364153	23.872819841111337	23.889544385286467	26.129298198238043
35-39	26.118178326794183	23.857322521253383	23.86504752857456	26.159451623377873
40-44	26.20039948804434	23.842288207740374	23.813685061559127	26.14362724265616
45-49	26.207965091634733	23.80144383803015	23.790241030680253	26.200350039654865
50-54	26.176347716574494	23.814864348312074	23.80942171098815	26.199366224125274
55-59	26.20884957194481	23.87893742060409	23.765823021775404	26.1463899856757
60-64	26.2197173300404	23.819375582058143	23.759480355143072	26.20142673275838
65-69	26.206946137624364	23.807903163779233	23.78648239764143	26.19866830095497
70-74	26.21812209565228	23.856049700550557	23.75910239324529	26.166725810551878
75-79	26.250522488549556	23.785700061557847	23.739393233244428	26.224384216648172
80-84	26.180824104409023	23.82893598699867	23.797237871949598	26.19300203664271
85-89	26.197325055461924	23.85729001518782	23.754679209344815	26.190705720005443
90-94	26.22267876632106	23.814212774883412	23.741519644551058	26.221588814244473
95-99	26.18244291106699	23.802176786167127	23.818798872972398	26.196581429793486
100-104	26.209820738012425	23.853341462764526	23.763402102269122	26.173435696953927
105-109	26.243409547742342	23.774330272911815	23.736857205108844	26.245402974237003
110-114	26.205610699943488	23.774298444432095	23.807065606680656	26.21302524894376
115-119	26.2316703054146	23.82186517838339	23.77556164660936	26.170902869592645
120-124	26.250923590209922	23.7780570667717	23.735802954860606	26.235216388157767
125-129	26.18484668692015	23.80504941195183	23.780589995935976	26.229513905192047
130-134	26.235039169519776	23.83287971573297	23.72919569279288	26.20288542195437
135-139	26.237401994223365	23.78162130582558	23.726258769397596	26.25471793055346
140-144	26.22412077932986	23.765024714181596	23.768321142569174	26.242533363919375
145-149	26.244377387171674	23.82492057370161	23.744334973697992	26.186367065428723
150	26.496494867649055	23.417199298053546	23.49401781545616	26.592288018841238
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	49.397540052508695
2	47.08233905467912
3	49.1265354068325
4	51.065489172313626
5	51.25479409003837
6	52.423302737574986
7	52.39489948197005
8	51.230124837953504
9	51.751530633689356
10-14	51.47879622953485
15-19	51.72120228814181
20-24	51.9116390245591
25-29	52.04319800911631
30-34	52.2376357736022
35-39	52.277629950172056
40-44	52.3440267307005
45-49	52.4083151312896
50-54	52.375713940699775
55-59	52.355239557620514
60-64	52.42114406279879
65-69	52.40561443857934
70-74	52.38484790620416
75-79	52.47490670519773
80-84	52.37382614105173
85-89	52.388030775467364
90-94	52.44426758056553
95-99	52.37902434086048
100-104	52.383256434966356
105-109	52.48881252197934
110-114	52.41863594888725
115-119	52.40257317500725
120-124	52.48613997836769
125-129	52.414360592112196
130-134	52.43792459147415
135-139	52.49211992477682
140-144	52.46665414324924
145-149	52.4307444526004
150	53.08878288649029
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	0.5
7	0.5
8	1.0
9	0.5
10	1.0
11	8.0
12	15.0
13	31.5
14	55.0
15	60.5
16	89.5
17	197.0
18	288.5
19	404.0
20	678.0
21	1061.5
22	1371.0
23	1747.5
24	2345.5
25	3193.0
26	4269.0
27	5444.5
28	6520.5
29	7684.5
30	9400.5
31	11272.5
32	13462.5
33	15849.0
34	18248.0
35	20721.5
36	24177.0
37	28503.0
38	33044.5
39	38143.0
40	43940.0
41	50492.0
42	57521.5
43	65574.5
44	75061.5
45	85931.0
46	98163.5
47	112675.0
48	129013.5
49	145920.5
50	161968.5
51	177011.0
52	190379.0
53	200041.5
54	206317.5
55	208588.0
56	204455.5
57	193653.0
58	179122.0
59	161442.0
60	141219.5
61	119828.5
62	98474.5
63	79137.5
64	62129.5
65	47301.0
66	34863.0
67	24963.5
68	17815.5
69	12644.5
70	8434.5
71	5488.0
72	3627.0
73	2413.5
74	1649.0
75	1204.5
76	809.0
77	526.0
78	502.5
79	473.5
80	436.0
81	361.5
82	347.5
83	362.5
84	232.0
85	140.0
86	68.5
87	23.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.06214014277947749
3	0.0
4	0.0
5	0.0
6	8.241398246615052E-5
7	0.00590633541007412
8	0.0
9	0.0
10-14	0.0015493828703636297
15-19	0.0
20-24	0.0
25-29	0.0012856581264719482
30-34	0.0
35-39	9.065538071276558E-4
40-44	0.0
45-49	0.0
50-54	5.4976131562720725E-6
55-59	9.360119646555274E-5
60-64	0.0011193698179518686
65-69	9.222054537905212E-4
70-74	0.0
75-79	3.3226506423348224E-4
80-84	2.8282003068985515E-4
85-89	3.3318791531873643E-5
90-94	2.3356061376170276E-4
95-99	3.897975285834351E-5
100-104	8.364370258552165E-5
105-109	5.5838274043416386E-6
110-114	1.677499492416612E-5
115-119	0.001629472510070812
120-124	1.6262339119903517E-4
125-129	5.61639831047506E-6
130-134	9.562861894554823E-5
135-139	2.8174310854947772E-5
140-144	1.693370460292523E-5
145-149	0.0013798326376111612
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	5553.0
55-59	5483.0
60-64	5234.0
65-69	5257.0
70-74	5008.0
75-79	5116.0
80-84	4924.0
85-89	5079.0
90-94	4903.0
95-99	4999.0
100-104	4830.0
105-109	5006.0
110-114	5005.0
115-119	5101.0
120-124	5490.0
125-129	5437.0
130-134	6020.0
135-139	5979.0
140-144	6063.0
145-149	61344.0
150-151	3478328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	58.37132688786287
#Duplication Level	Relative count
1	100.0
2	53.899772616695294
3	23.8951057977778
4	9.966140739343269
5	4.541708452899882
6	2.2877342097685456
7	1.4695514889138228
8	0.8926532354956261
9	0.5977499975176496
10++	1.0376225039965843
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1394275	3.444542	4.1422625	8
AAAAA	1409180	3.4106271	4.8650784	1
>>END_MODULE