##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	ICCR100_2P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4433327	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.226799647307764	37.0	37.0	37.0	37.0	37.0
2	36.255758485670015	37.0	37.0	37.0	37.0	37.0
3	36.34073981007943	37.0	37.0	37.0	37.0	37.0
4	36.35226208217892	37.0	37.0	37.0	37.0	37.0
5	36.395214023238076	37.0	37.0	37.0	37.0	37.0
6	36.398073275443025	37.0	37.0	37.0	37.0	37.0
7	36.3868699511676	37.0	37.0	37.0	37.0	37.0
8	36.377342569136	37.0	37.0	37.0	37.0	37.0
9	36.369491129348226	37.0	37.0	37.0	37.0	37.0
10-14	36.38851038960131	37.0	37.0	37.0	37.0	37.0
15-19	36.399452781173146	37.0	37.0	37.0	37.0	37.0
20-24	36.39961108215117	37.0	37.0	37.0	37.0	37.0
25-29	36.358973339886724	37.0	37.0	37.0	37.0	37.0
30-34	36.323888763450114	37.0	37.0	37.0	37.0	37.0
35-39	36.30887362921796	37.0	37.0	37.0	37.0	37.0
40-44	36.28965257920294	37.0	37.0	37.0	37.0	37.0
45-49	36.26282464614048	37.0	37.0	37.0	37.0	37.0
50-54	36.22916699693586	37.0	37.0	37.0	37.0	37.0
55-59	36.1789318709906	37.0	37.0	37.0	37.0	37.0
60-64	36.1780087318527	37.0	37.0	37.0	37.0	37.0
65-69	36.125819044065224	37.0	37.0	37.0	37.0	37.0
70-74	36.12520210936723	37.0	37.0	37.0	37.0	37.0
75-79	36.09094583350394	37.0	37.0	37.0	37.0	37.0
80-84	36.03753323948631	37.0	37.0	37.0	37.0	37.0
85-89	36.00406296650941	37.0	37.0	37.0	37.0	37.0
90-94	35.97732239625233	37.0	37.0	37.0	37.0	37.0
95-99	35.95029352261473	37.0	37.0	37.0	37.0	37.0
100-104	35.896009884043174	37.0	37.0	37.0	37.0	37.0
105-109	35.821189571745585	37.0	37.0	37.0	37.0	37.0
110-114	35.82332978705942	37.0	37.0	37.0	37.0	37.0
115-119	35.77204818573965	37.0	37.0	37.0	37.0	37.0
120-124	35.728273380916434	37.0	37.0	37.0	37.0	37.0
125-129	35.70096494706277	37.0	37.0	37.0	37.0	37.0
130-134	35.58885485063247	37.0	37.0	37.0	37.0	37.0
135-139	35.52852820114673	37.0	37.0	37.0	37.0	37.0
140-144	35.45256846038323	37.0	37.0	37.0	37.0	37.0
145-149	35.37960874571064	37.0	37.0	37.0	34.6	37.0
150	36.19124312935791	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	3.0
26	40.0
27	314.0
28	1579.0
29	5511.0
30	14926.0
31	34477.0
32	68226.0
33	132479.0
34	287355.0
35	888336.0
36	2877510.0
37	122571.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.64963490473632	29.695565538328516	21.006070367342627	25.648729189592533
2	28.246303509756892	29.20790638723469	24.245177493110702	18.300612609897712
3	22.363046984803965	26.108044816003872	25.08784035105013	26.44106784814204
4	24.321914444840186	25.238291693800164	23.884816076053042	26.554977785306612
5	25.355855771523284	24.83949413160816	24.14444050709546	25.660209589773096
6	25.55370365483097	23.492680255241996	24.30153906258635	26.652077027340688
7	26.54137626211647	24.07286897627899	23.70296619220734	25.682788569397204
8	24.80766701847168	23.82323704071457	25.161532185647484	26.20756375516627
9	25.567367351878172	23.51310877812532	24.93102809695743	25.988495773039073
10-14	25.687535723743306	24.388492973447022	24.28750784399794	25.636463458811736
15-19	25.693531472679958	24.101696686125653	24.338922728054822	25.865849113139568
20-24	25.849936636717285	24.035781183268195	24.174309275706996	25.939972904307524
25-29	25.96193818752849	24.003097088248925	24.012832444015952	26.022132280206637
30-34	26.072181003346532	23.899437973637365	23.888195641836994	26.14018538117911
35-39	26.081841691826547	23.888327357744714	23.892004064678463	26.137826885750282
40-44	26.1435458831067	23.899683654074018	23.819436640521126	26.13733382229816
45-49	26.15226602370609	23.879155086570233	23.76423457498674	26.20434431473694
50-54	26.14035813241801	23.87144333067447	23.81118995407452	26.177008582833
55-59	26.193483429042868	23.904709307164286	23.770801897545564	26.131005366247283
60-64	26.176433201694948	23.824620418987557	23.756336735357447	26.242609643960048
65-69	26.17614127456736	23.83907531104331	23.811107485484083	26.173675928905244
70-74	26.19084498504855	23.89391440541168	23.750520577096246	26.164720032443523
75-79	26.21518553895014	23.82887184608442	23.741645835140286	26.214296779825148
80-84	26.157562696066122	23.855403542260326	23.771386701630533	26.215647060043025
85-89	26.18175075093596	23.885157720800244	23.75810757366932	26.174983954594477
90-94	26.201741431873426	23.824240543535133	23.740800237507575	26.233217787083866
95-99	26.170912707379884	23.821518872378427	23.806933297709314	26.20063512253238
100-104	26.19634631849847	23.881184224373694	23.767252398769493	26.155217058358343
105-109	26.201935155500255	23.819116395907958	23.709532977618636	26.26941547097315
110-114	26.18658317785398	23.790325004955516	23.771969022093682	26.25112279509682
115-119	26.22142072694122	23.86051286692651	23.697628641213576	26.220437764918696
120-124	26.238146244019926	23.78071452640036	23.714038274790106	26.26710095478961
125-129	26.18087838292255	23.78789731059891	23.76448199367255	26.266742312805984
130-134	26.247574161676873	23.80844078860562	23.68244045236168	26.261544597355822
135-139	26.237250494236307	23.755158853733715	23.688423003829644	26.31916764820033
140-144	26.238557727796202	23.735170099266007	23.70890263038783	26.31736954254996
145-149	26.28486499628141	23.74671990258552	23.663190879520425	26.30522422161265
150	26.743693971094835	22.899239220704228	23.39889561220132	26.95817119599962
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	49.29836409432885
2	46.54691611965461
3	48.804114832946
4	50.876892230146794
5	51.016065361296384
6	52.20578068217166
7	52.22416483151366
8	51.015230773637946
9	51.555863124917245
10-14	51.323999182555035
15-19	51.559380585819525
20-24	51.78990954102481
25-29	51.98407046773512
30-34	52.212366384525644
35-39	52.219668577576826
40-44	52.28087970540486
45-49	52.35661033844302
50-54	52.31736671525101
55-59	52.32448879529015
60-64	52.41904284565499
65-69	52.349817203472604
70-74	52.35556501749207
75-79	52.429482318775285
80-84	52.37320975610914
85-89	52.35673470553044
90-94	52.43495921895729
95-99	52.37154782991226
100-104	52.35156337685682
105-109	52.47135062647341
110-114	52.4377059729508
115-119	52.44185849185992
120-124	52.505247198809535
125-129	52.44762069572854
130-134	52.5091187590327
135-139	52.55641814243665
140-144	52.55592727034616
145-149	52.590089217894054
150	53.701865167094454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	16.0
2	3.0
3	1.0
4	0.5
5	0.0
6	1.0
7	3.0
8	2.5
9	1.0
10	1.0
11	4.5
12	16.0
13	48.0
14	71.5
15	94.0
16	130.5
17	235.5
18	414.0
19	574.5
20	916.0
21	1329.0
22	1666.5
23	2201.0
24	3021.0
25	4020.0
26	5346.5
27	6864.5
28	8415.0
29	10082.0
30	12090.0
31	14635.0
32	17201.0
33	19951.5
34	23284.0
35	26702.0
36	30975.0
37	35736.0
38	41294.0
39	47614.5
40	54323.0
41	62609.0
42	71152.0
43	80963.5
44	93126.5
45	106127.5
46	119949.5
47	137211.0
48	156899.0
49	177410.0
50	196838.0
51	215062.5
52	231434.0
53	242123.5
54	249004.5
55	251558.0
56	246650.0
57	234080.0
58	216840.5
59	195720.5
60	170786.5
61	145014.5
62	120034.5
63	96876.0
64	76197.0
65	57999.5
66	42767.5
67	31116.0
68	22448.5
69	15680.0
70	10551.0
71	6976.5
72	4550.0
73	2965.5
74	2105.5
75	1526.5
76	987.0
77	658.0
78	615.0
79	587.0
80	534.0
81	415.0
82	355.5
83	335.0
84	199.0
85	107.5
86	39.5
87	17.0
88	4.5
89	3.5
90	1.5
91	0.0
92	1.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.5
99	18.0
100	96.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03315794210533082
2	0.0
3	0.0
4	0.0
5	0.0
6	9.924826208398342E-4
7	0.0
8	0.0
9	0.0
10-14	2.2556423200905325E-5
15-19	5.864670032235384E-5
20-24	5.413541568217278E-5
25-29	3.609027712144852E-5
30-34	0.001872183125675142
35-39	2.7067707841086394E-4
40-44	3.5188020193412307E-4
45-49	4.0601561761629584E-5
50-54	9.029427717580088E-6
55-59	9.49857433183975E-5
60-64	1.2688749108218854E-4
65-69	1.3620675713549744E-4
70-74	2.7294040196205935E-5
75-79	4.101963509661856E-5
80-84	2.465839560149019E-4
85-89	6.4053104782095E-4
90-94	1.3753139039376246E-5
95-99	5.0532674105505056E-5
100-104	9.207355351066332E-6
105-109	4.614036535879186E-5
110-114	2.7749021569499457E-5
115-119	1.2518840275037993E-4
120-124	2.3241855867769085E-5
125-129	1.6778463276394842E-4
130-134	1.8695795338998006E-5
135-139	3.0006193465870065E-4
140-144	9.408118340581035E-6
145-149	1.416784724189323E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	8425.0
55-59	8149.0
60-64	8379.0
65-69	8298.0
70-74	8677.0
75-79	8259.0
80-84	8310.0
85-89	8678.0
90-94	8885.0
95-99	9117.0
100-104	9503.0
105-109	9998.0
110-114	10754.0
115-119	10908.0
120-124	10848.0
125-129	12222.0
130-134	12715.0
135-139	13813.0
140-144	14696.0
145-149	152282.0
150-151	4090411.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	62.000717921942304
#Duplication Level	Relative count
1	100.0
2	58.71763493494783
3	28.802224226029455
4	12.836532268452917
5	5.872944308471811
6	3.0765597492378376
7	1.8442183004852075
8	1.1550517411653571
9	0.833011292885053
10++	1.5554553651938683
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1696075	3.4542465	4.115403	7
AAAAA	1718180	3.4007037	5.0291567	1
>>END_MODULE