##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	An98_2P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3348717	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.23149552500256	37.0	37.0	37.0	37.0	37.0
2	36.38199196886449	37.0	37.0	37.0	37.0	37.0
3	36.44674273759174	37.0	37.0	37.0	37.0	37.0
4	36.45211613880779	37.0	37.0	37.0	37.0	37.0
5	36.483286285463954	37.0	37.0	37.0	37.0	37.0
6	36.467393631650566	37.0	37.0	37.0	37.0	37.0
7	36.4779069118113	37.0	37.0	37.0	37.0	37.0
8	36.46447908258596	37.0	37.0	37.0	37.0	37.0
9	36.4831668367318	37.0	37.0	37.0	37.0	37.0
10-14	36.4773788886908	37.0	37.0	37.0	37.0	37.0
15-19	36.478507081966015	37.0	37.0	37.0	37.0	37.0
20-24	36.479516125130914	37.0	37.0	37.0	37.0	37.0
25-29	36.44351845796464	37.0	37.0	37.0	37.0	37.0
30-34	36.41470903632644	37.0	37.0	37.0	37.0	37.0
35-39	36.40119986251451	37.0	37.0	37.0	37.0	37.0
40-44	36.38647917993667	37.0	37.0	37.0	37.0	37.0
45-49	36.36231231244683	37.0	37.0	37.0	37.0	37.0
50-54	36.33464427623739	37.0	37.0	37.0	37.0	37.0
55-59	36.29124095737207	37.0	37.0	37.0	37.0	37.0
60-64	36.2921847564638	37.0	37.0	37.0	37.0	37.0
65-69	36.24991208560628	37.0	37.0	37.0	37.0	37.0
70-74	36.248727228827626	37.0	37.0	37.0	37.0	37.0
75-79	36.21933930899301	37.0	37.0	37.0	37.0	37.0
80-84	36.17391073583545	37.0	37.0	37.0	37.0	37.0
85-89	36.14472100564669	37.0	37.0	37.0	37.0	37.0
90-94	36.12191259530286	37.0	37.0	37.0	37.0	37.0
95-99	36.09764327010627	37.0	37.0	37.0	37.0	37.0
100-104	36.05267773382873	37.0	37.0	37.0	37.0	37.0
105-109	35.985566071180855	37.0	37.0	37.0	37.0	37.0
110-114	35.98713320538568	37.0	37.0	37.0	37.0	37.0
115-119	35.94142304434907	37.0	37.0	37.0	37.0	37.0
120-124	35.899661515709035	37.0	37.0	37.0	37.0	37.0
125-129	35.877377944128305	37.0	37.0	37.0	37.0	37.0
130-134	35.778088284845595	37.0	37.0	37.0	37.0	37.0
135-139	35.719684139921796	37.0	37.0	37.0	37.0	37.0
140-144	35.64995518018067	37.0	37.0	37.0	37.0	37.0
145-149	35.58015470434249	37.0	37.0	37.0	37.0	37.0
150	36.25930216553496	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	28.0
27	219.0
28	1084.0
29	3798.0
30	10175.0
31	22793.0
32	44600.0
33	83449.0
34	169206.0
35	521017.0
36	2354995.0
37	137351.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	23.895133458062077	29.093472173969413	21.22337328616225	25.78802108180626
2	28.456838843055415	28.883002057205793	23.647116194052828	19.013042905685968
3	22.676535520917415	25.88654102451775	24.802036123088335	26.634887331476502
4	24.708029970881384	24.90067091366634	23.676022787234633	26.71527632821764
5	25.592965903060783	24.646573598187008	23.91506956246228	25.84539093628993
6	25.784451590882014	23.341964602737143	24.07019249691744	26.803391309463407
7	26.703152281903787	23.980676778599086	23.53411769343304	25.78205324606409
8	24.923873829887686	23.837607059658968	25.013908311750438	26.22461079870291
9	25.646329624151576	23.475348917212173	24.911182402096085	25.967139056540162
10-14	25.6976584458043	24.37683577825053	24.29441612845043	25.631089647494743
15-19	25.749905336851498	24.045317668025596	24.30189959375474	25.90287740136817
20-24	25.91760720156836	23.97510686935138	24.142042544677587	25.965243384402676
25-29	26.042382923654987	23.960803371655874	23.94067028544607	26.05614341924306
30-34	26.101836211523217	23.863134805502245	23.86054272048759	26.17448626248695
35-39	26.150100414858557	23.848496058598844	23.805094239939013	26.196309286603586
40-44	26.21257013224259	23.853661353833807	23.76959314534728	26.164175368576327
45-49	26.21949271186373	23.804143211658246	23.737060787653487	26.239303288824534
50-54	26.221312891113147	23.78677307713225	23.776368294637624	26.215545737116987
55-59	26.237889093057664	23.824776182191282	23.70679020068092	26.230544524070133
60-64	26.257277542701225	23.780651321299175	23.68100449468879	26.28106664131081
65-69	26.217185308548558	23.79316042882151	23.74885929689096	26.240794965738978
70-74	26.261119405507287	23.82785508277965	23.71028487351911	26.20074063819395
75-79	26.25385195819131	23.78500870487286	23.690578167035618	26.270561169900216
80-84	26.226110113640104	23.782969077782187	23.735256569503836	26.255664239073873
85-89	26.25412384816082	23.83260830461949	23.702683787483053	26.210584059736636
90-94	26.249498233886158	23.814381063139592	23.6783618238851	26.25775887908915
95-99	26.195228294861728	23.756273394804737	23.785722153207377	26.26277615712616
100-104	26.24359071903387	23.81249513844642	23.711866672018296	26.232047470501413
105-109	26.2595630378092	23.762186104162666	23.680633664946665	26.297617193081468
110-114	26.223743176137155	23.77428545015907	23.716807871259483	26.28516350244429
115-119	26.25714926144219	23.80852359206665	23.680902532033336	26.25342461445782
120-124	26.26751269380262	23.75756257943282	23.668378180761852	26.306546546002714
125-129	26.232589123798167	23.778102813769745	23.717298549550385	26.2720095128817
130-134	26.2772648563577	23.78212223640511	23.676330145542	26.264282761695192
135-139	26.26331851008083	23.717337441013274	23.68478327274949	26.33456077615641
140-144	26.260523411230928	23.720803219734588	23.695242714592325	26.32343065444216
145-149	26.2652752177008	23.768613981892408	23.655193834085217	26.310916966321578
150	26.676339695356948	23.00794870618462	23.448237189290595	26.86747440916784
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	49.68315453986834
2	47.469881748741386
3	49.31142285239392
4	51.423306299099025
5	51.43835683935071
6	52.587842900345414
7	52.48520552796787
8	51.148484628590595
9	51.613468680691746
10-14	51.32874809329905
15-19	51.65278273821966
20-24	51.88285058597104
25-29	52.098526342898055
30-34	52.276322474010165
35-39	52.346409701462136
40-44	52.37674550081891
45-49	52.45879600068827
50-54	52.436858628230134
55-59	52.46843361712779
60-64	52.53834418401203
65-69	52.45798027428753
70-74	52.461860043701236
75-79	52.52441312809153
80-84	52.481774352713984
85-89	52.464707907897456
90-94	52.5072571129753
95-99	52.45800445198788
100-104	52.47563818953528
105-109	52.557180230890665
110-114	52.50890667858145
115-119	52.510573875900015
120-124	52.57405923980533
125-129	52.504598636679866
130-134	52.54154761805289
135-139	52.59787928623724
140-144	52.58395406567309
145-149	52.576192184022375
150	53.54381410452479
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18.0
1	11.5
2	2.5
3	0.0
4	0.5
5	1.0
6	1.0
7	1.0
8	1.0
9	1.5
10	2.5
11	7.0
12	17.5
13	30.0
14	54.5
15	73.0
16	92.5
17	157.0
18	262.5
19	420.5
20	686.0
21	979.0
22	1262.5
23	1637.5
24	2188.0
25	2963.5
26	3913.5
27	5029.5
28	6133.5
29	7231.0
30	8787.0
31	10595.5
32	12557.0
33	14480.5
34	16710.0
35	19382.5
36	22546.5
37	26079.5
38	29943.5
39	34597.0
40	39748.0
41	45590.0
42	52155.5
43	59224.5
44	68024.5
45	77916.0
46	88762.0
47	102097.0
48	117149.5
49	132991.0
50	148140.5
51	162651.5
52	175238.0
53	184088.5
54	189404.5
55	191116.5
56	188394.0
57	179760.5
58	166641.5
59	150273.5
60	131243.5
61	111370.0
62	91957.0
63	74167.5
64	58254.0
65	44351.0
66	32590.5
67	23416.5
68	16871.5
69	11858.0
70	8003.5
71	5185.0
72	3281.0
73	2270.0
74	1612.0
75	1059.5
76	688.5
77	476.5
78	435.0
79	380.0
80	362.5
81	308.5
82	269.5
83	283.0
84	185.5
85	98.5
86	38.5
87	11.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	2.0
99	12.5
100	68.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03541654908432095
2	0.0
3	0.0
4	0.0
5	0.0
6	0.001194487321562258
7	0.0
8	0.0
9	0.0
10-14	2.986218303905645E-5
15-19	2.986218303905645E-5
20-24	5.97243660781129E-5
25-29	1.1944873215622582E-5
30-34	0.0018335380385980662
35-39	2.8070452056713064E-4
40-44	2.2695259109682903E-4
45-49	2.3889746431245163E-5
50-54	1.195264934058429E-5
55-59	1.3168729009345248E-4
60-64	1.1990458712384207E-4
65-69	1.0808071467772132E-4
70-74	2.4055279995641187E-5
75-79	4.818804226982786E-5
80-84	1.9304555790709176E-4
85-89	6.223383705683013E-4
90-94	6.051754361227039E-6
95-99	6.0619062476491174E-5
100-104	6.0721977020132124E-6
105-109	5.47453980106009E-5
110-114	6.0938905213067395E-6
115-119	1.1601346000374906E-4
120-124	6.118154908867941E-5
125-129	1.532672187597114E-4
130-134	1.2287830636338828E-5
135-139	3.0176871571114476E-4
140-144	1.2348068592286223E-5
145-149	1.7956971010699445E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	5392.0
55-59	5214.0
60-64	5269.0
65-69	5038.0
70-74	5440.0
75-79	5047.0
80-84	5088.0
85-89	5316.0
90-94	5436.0
95-99	5548.0
100-104	5637.0
105-109	5900.0
110-114	6211.0
115-119	6638.0
120-124	6448.0
125-129	6971.0
130-134	7388.0
135-139	8051.0
140-144	8421.0
145-149	95640.0
150-151	3138624.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	56.22715410116299
#Duplication Level	Relative count
1	100.0
2	51.73080568720379
3	21.649289099526065
4	8.660663507109005
5	3.591469194312796
6	1.6824644549763033
7	0.9829383886255925
8	0.5469194312796208
9	0.3175355450236967
10++	0.41137440758293836
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1264370	3.4403892	4.2598634	8
AAAAA	1278885	3.3858747	4.8378625	1
>>END_MODULE