##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	An100_2P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5488876	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.13756732708117	37.0	37.0	37.0	37.0	37.0
2	36.18241694656611	37.0	37.0	37.0	37.0	37.0
3	36.273511006625036	37.0	37.0	37.0	37.0	37.0
4	36.296595878646194	37.0	37.0	37.0	37.0	37.0
5	36.28384973535565	37.0	37.0	37.0	37.0	37.0
6	36.28328313483489	37.0	37.0	37.0	37.0	37.0
7	36.29467052999558	37.0	37.0	37.0	37.0	37.0
8	36.29808252181321	37.0	37.0	37.0	37.0	37.0
9	36.303745065474246	37.0	37.0	37.0	37.0	37.0
10-14	36.30110150056223	37.0	37.0	37.0	37.0	37.0
15-19	36.30287669096551	37.0	37.0	37.0	37.0	37.0
20-24	36.29093482891579	37.0	37.0	37.0	37.0	37.0
25-29	36.25695818233096	37.0	37.0	37.0	37.0	37.0
30-34	36.22952571710492	37.0	37.0	37.0	37.0	37.0
35-39	36.21058205723722	37.0	37.0	37.0	37.0	37.0
40-44	36.19081644402242	37.0	37.0	37.0	37.0	37.0
45-49	36.15547977400109	37.0	37.0	37.0	37.0	37.0
50-54	36.10967411331039	37.0	37.0	37.0	37.0	37.0
55-59	36.069838416159165	37.0	37.0	37.0	37.0	37.0
60-64	36.049763261257915	37.0	37.0	37.0	37.0	37.0
65-69	36.034325801075724	37.0	37.0	37.0	37.0	37.0
70-74	36.001103215743626	37.0	37.0	37.0	37.0	37.0
75-79	35.98092297512572	37.0	37.0	37.0	37.0	37.0
80-84	35.94604722964222	37.0	37.0	37.0	37.0	37.0
85-89	35.89526673760416	37.0	37.0	37.0	37.0	37.0
90-94	35.86771785397828	37.0	37.0	37.0	37.0	37.0
95-99	35.859860572158325	37.0	37.0	37.0	37.0	37.0
100-104	35.81962557314502	37.0	37.0	37.0	37.0	37.0
105-109	35.75973464711954	37.0	37.0	37.0	37.0	37.0
110-114	35.71973385030827	37.0	37.0	37.0	37.0	37.0
115-119	35.674595637612306	37.0	37.0	37.0	37.0	37.0
120-124	35.65934763675534	37.0	37.0	37.0	37.0	37.0
125-129	35.606500704056224	37.0	37.0	37.0	37.0	37.0
130-134	35.518502331261764	37.0	37.0	37.0	37.0	37.0
135-139	35.50864425369764	37.0	37.0	37.0	37.0	37.0
140-144	35.45576825430059	37.0	37.0	37.0	37.0	37.0
145-149	35.415800193119004	37.0	37.0	37.0	37.0	37.0
150	36.15552003032758	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
24	1.0
25	8.0
26	64.0
27	443.0
28	2285.0
29	8290.0
30	22583.0
31	52022.0
32	102510.0
33	194764.0
34	409407.0
35	1190565.0
36	3381030.0
37	124904.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.859061981522363	29.11332733484305	20.95421950754267	26.07339117609192
2	28.55358729182441	29.027309051980772	23.878167406223056	18.54093624997176
3	22.548314081061406	26.070601704246915	24.880722391979706	26.50036182271197
4	24.51186727483004	25.115870717429218	23.75253148367717	26.619730524063577
5	25.414065585860808	24.792976316621797	24.050985070537084	25.741973026980308
6	25.7282006211841	23.31087334519734	24.202131148257784	26.758794885360782
7	26.62291879065951	23.949238423312895	23.661857181688926	25.765985604338663
8	24.875074605438343	23.730924145489894	25.14642342075135	26.247577828320406
9	25.65788167211922	23.39214996464662	24.991423548794316	25.95854481443985
10-14	25.722597187266615	24.331319220111574	24.317582323726107	25.628501268895704
15-19	25.75450392224529	23.99734488222843	24.332309917117023	25.915841278409257
20-24	25.878195973971348	23.99396652467682	24.179276974062585	25.94856052728925
25-29	26.031610114712013	23.943430312508426	23.987883858188816	26.037075714590745
30-34	26.11887026278718	23.83890409285772	23.887661166432004	26.1545644779231
35-39	26.141545689811473	23.807810039568047	23.879369348202882	26.1712749224176
40-44	26.195744942579672	23.860286571635424	23.81288159683858	26.13108688894632
45-49	26.218016220442948	23.765601554853856	23.77291088375835	26.24347134094485
50-54	26.214584208149645	23.74091914228286	23.8206892607969	26.22380738877059
55-59	26.238746040349604	23.82126045810053	23.76342498292238	26.17656851862749
60-64	26.250258166267393	23.767188544350994	23.728940197759666	26.253613091621947
65-69	26.23100540917672	23.783294949448084	23.781224774328887	26.204474867046308
70-74	26.25667761089427	23.820369977036176	23.728056335636584	26.194896076432972
75-79	26.24436646103028	23.754440943501905	23.727536579594172	26.273656015873648
80-84	26.212359375585947	23.779872672085084	23.768035088607306	26.239732863721667
85-89	26.233629250762895	23.829014097086006	23.737548440473898	26.199808211677194
90-94	26.253546507461394	23.747127082622708	23.73758177300118	26.26174463691472
95-99	26.21903558620739	23.75249553716042	23.77823768093342	26.25023119569877
100-104	26.25069958150408	23.807433553181916	23.733082508254764	26.20878435705924
105-109	26.264449478621703	23.739756330733783	23.696326208704747	26.299467981939767
110-114	26.22624525707296	23.746777605910097	23.771539446556744	26.255437690460198
115-119	26.264572397175684	23.77727405172857	23.71469795531628	26.24345559577947
120-124	26.268244407013707	23.732554736425154	23.700046184459495	26.29915467210165
125-129	26.226654983446085	23.729385487601046	23.742240256538842	26.301719272414033
130-134	26.282863506818245	23.76132922263578	23.694361232250465	26.26144603829551
135-139	26.29241618475818	23.68204717478607	23.688740506713003	26.33679613374274
140-144	26.241590050339596	23.69019552104283	23.758516874813022	26.309697553804547
145-149	26.290159680118286	23.7178197245054	23.686189686667454	26.30583090870886
150	26.644517044658667	23.001691024694207	23.429841549138363	26.92395038150876
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	49.93245315761428
2	47.09452354179617
3	49.04867590377337
4	51.131597798893615
5	51.156038612841115
6	52.48699550654487
7	52.38890439499817
8	51.12265243375875
9	51.616426486559064
10-14	51.351098456162326
15-19	51.670345200654545
20-24	51.8267565012606
25-29	52.06868582930275
30-34	52.27343474071028
35-39	52.312820612229075
40-44	52.326831831526
45-49	52.4614875613878
50-54	52.43839159692024
55-59	52.41531455897709
60-64	52.50387125788934
65-69	52.435480276223025
70-74	52.45157368732723
75-79	52.51802247690392
80-84	52.45209223930761
85-89	52.43343746244009
90-94	52.51529114437611
95-99	52.46926678190616
100-104	52.459483938563324
105-109	52.56391746056147
110-114	52.48168294753316
115-119	52.50802799295515
120-124	52.567399079115354
125-129	52.52837425586011
130-134	52.544309545113755
135-139	52.629212318500926
140-144	52.55128760414415
145-149	52.59599058882715
150	53.56846742616743
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	13.5
2	2.5
3	0.5
4	0.0
5	0.5
6	1.5
7	3.0
8	4.0
9	3.0
10	5.0
11	14.5
12	26.5
13	45.0
14	73.5
15	105.0
16	164.0
17	307.0
18	505.0
19	695.0
20	1094.5
21	1602.5
22	2069.0
23	2681.0
24	3660.0
25	4873.0
26	6327.0
27	8203.5
28	10005.0
29	12025.0
30	14553.0
31	17484.0
32	20770.0
33	24001.0
34	27667.5
35	31654.5
36	36832.5
37	43017.5
38	49650.0
39	57236.0
40	66247.0
41	76407.0
42	87193.0
43	99118.0
44	113311.0
45	129792.0
46	147994.0
47	169753.5
48	194018.0
49	219286.5
50	243815.0
51	266567.0
52	286516.5
53	300929.0
54	310550.5
55	313755.5
56	307221.5
57	292153.5
58	270704.5
59	244212.0
60	213913.5
61	182193.5
62	150807.5
63	121511.5
64	95571.0
65	72717.0
66	53513.0
67	38723.5
68	27857.0
69	19400.0
70	13092.0
71	8794.5
72	5827.0
73	3891.0
74	2704.0
75	1855.5
76	1224.0
77	816.5
78	746.0
79	706.5
80	626.5
81	539.0
82	500.0
83	479.0
84	310.5
85	166.5
86	67.5
87	22.0
88	2.5
89	0.0
90	1.0
91	1.5
92	0.5
93	0.5
94	1.0
95	1.0
96	0.5
97	1.5
98	3.0
99	26.5
100	105.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09608524586818867
2	0.0
3	0.0
4	0.0
5	0.0024048639466440853
6	7.287466504982076E-5
7	0.0
8	0.0
9	1.6396799636209672E-4
10-14	1.6032426310960568E-4
15-19	8.380586480729388E-5
20-24	5.975722534085303E-4
25-29	0.0
30-34	5.72066120641093E-4
35-39	4.372479902989246E-5
40-44	1.0931199757473115E-5
45-49	0.0
50-54	4.011662559514382E-5
55-59	5.774742358773193E-4
60-64	7.32516399302454E-6
65-69	1.908668452690936E-4
70-74	0.0
75-79	0.0
80-84	4.803012212803885E-5
85-89	5.1838657953427554E-5
90-94	3.7112399950536596E-6
95-99	0.0
100-104	8.726047093954095E-4
105-109	7.51432291632592E-4
110-114	3.5234708136668686E-4
115-119	7.254384307436146E-4
120-124	0.0
125-129	0.0
130-134	3.982336177214415E-4
135-139	7.610382510001565E-6
140-144	0.0
145-149	3.834408610149197E-6
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	12107.0
55-59	11477.0
60-64	11652.0
65-69	12000.0
70-74	11929.0
75-79	11660.0
80-84	11886.0
85-89	12060.0
90-94	12550.0
95-99	12914.0
100-104	13408.0
105-109	13750.0
110-114	14548.0
115-119	15063.0
120-124	15555.0
125-129	16288.0
130-134	17047.0
135-139	17945.0
140-144	19368.0
145-149	192626.0
150-151	5033043.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	61.376750979784376
#Duplication Level	Relative count
1	100.0
2	61.30032321189068
3	29.739891383214967
4	13.084584771662891
5	5.915657087575031
6	3.038631516457422
7	1.9953387128691102
8	1.3961874189221872
9	1.035597282381654
10++	2.3658230909610607
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	2111510	3.430965	5.0107503	1
TTTTT	2065050	3.4157045	4.234415	8
>>END_MODULE