##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	An100_1P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	5488876	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.4921033012952	37.0	37.0	37.0	37.0	37.0
2	36.47383726650046	37.0	37.0	37.0	37.0	37.0
3	36.52658322031687	37.0	37.0	37.0	37.0	37.0
4	36.55968580816911	37.0	37.0	37.0	37.0	37.0
5	36.580043710224096	37.0	37.0	37.0	37.0	37.0
6	36.586974819616984	37.0	37.0	37.0	37.0	37.0
7	36.56905238886796	37.0	37.0	37.0	37.0	37.0
8	36.580821100713514	37.0	37.0	37.0	37.0	37.0
9	36.578920347262354	37.0	37.0	37.0	37.0	37.0
10-14	36.584617324202625	37.0	37.0	37.0	37.0	37.0
15-19	36.5749906538242	37.0	37.0	37.0	37.0	37.0
20-24	36.55683043304312	37.0	37.0	37.0	37.0	37.0
25-29	36.53058123375351	37.0	37.0	37.0	37.0	37.0
30-34	36.51515479671976	37.0	37.0	37.0	37.0	37.0
35-39	36.50301752125572	37.0	37.0	37.0	37.0	37.0
40-44	36.48878393317684	37.0	37.0	37.0	37.0	37.0
45-49	36.47001313201464	37.0	37.0	37.0	37.0	37.0
50-54	36.441810773988706	37.0	37.0	37.0	37.0	37.0
55-59	36.432554805762145	37.0	37.0	37.0	37.0	37.0
60-64	36.44015966762562	37.0	37.0	37.0	37.0	37.0
65-69	36.42585152482135	37.0	37.0	37.0	37.0	37.0
70-74	36.420326838656266	37.0	37.0	37.0	37.0	37.0
75-79	36.4091843461306	37.0	37.0	37.0	37.0	37.0
80-84	36.39843855420858	37.0	37.0	37.0	37.0	37.0
85-89	36.37258951541498	37.0	37.0	37.0	37.0	37.0
90-94	36.37239839670521	37.0	37.0	37.0	37.0	37.0
95-99	36.33661811529716	37.0	37.0	37.0	37.0	37.0
100-104	36.32019221258024	37.0	37.0	37.0	37.0	37.0
105-109	36.31973018457383	37.0	37.0	37.0	37.0	37.0
110-114	36.29553298604486	37.0	37.0	37.0	37.0	37.0
115-119	36.27594015054302	37.0	37.0	37.0	37.0	37.0
120-124	36.26527324475302	37.0	37.0	37.0	37.0	37.0
125-129	36.23712418702842	37.0	37.0	37.0	37.0	37.0
130-134	36.195160942410766	37.0	37.0	37.0	37.0	37.0
135-139	36.16266545965043	37.0	37.0	37.0	37.0	37.0
140-144	36.181109345402625	37.0	37.0	37.0	37.0	37.0
145-149	36.117583570370655	37.0	37.0	37.0	37.0	37.0
150	36.497943117424796	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	9.0
27	103.0
28	754.0
29	3761.0
30	12747.0
31	32519.0
32	65289.0
33	115331.0
34	201664.0
35	471104.0
36	4004757.0
37	580838.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.98278772659019	28.94227486366056	21.069184681594074	26.005752728155173
2	28.25863102777712	28.86170490022581	24.232452263486238	18.647211808510832
3	22.459947804426204	25.835023506956617	25.249754581688403	26.455274106928776
4	24.41822333024102	25.127457789172137	23.881628952812925	26.57268992777392
5	25.402332063006	24.753437590296297	24.116858940467573	25.72737140623013
6	25.63778642726644	23.429755860449625	24.26656473841046	26.665892973873472
7	26.528845004649483	24.058534640823908	23.714906616838995	25.697713737687618
8	24.851914045014343	23.89823921959936	25.13601608198987	26.113830653396423
9	25.588991261598913	23.5301726619439	24.978064725820005	25.902771350637178
10-14	25.71741266934836	24.36718408285344	24.276855724879425	25.63854752291877
15-19	25.741168137155952	24.087244820250994	24.302713342403802	25.868873700189255
20-24	25.8771303997394	24.060437874712417	24.137590282600662	25.924841442947518
25-29	26.005852224499364	24.001182032020168	23.98397989525316	26.008985848227304
30-34	26.07809686354729	23.898109558313944	23.882004257337933	26.141789320800836
35-39	26.106217262210794	23.892496491608682	23.866808005567076	26.134478240613447
40-44	26.164434573965135	23.910775806313502	23.804225642072048	26.120563977649315
45-49	26.181323216966916	23.84144465606848	23.767516933252583	26.209715193712025
50-54	26.14227942771973	23.857541876011776	23.824851399465295	26.175327296803196
55-59	26.193055428675105	23.910861945296567	23.763534106080648	26.132548519947683
60-64	26.19812690060498	23.83746877290304	23.744748934432895	26.21965539205909
65-69	26.167140275438612	23.85280949506582	23.793069998803013	26.18698023069256
70-74	26.208531912194626	23.896749046984567	23.7221720354904	26.172547005330404
75-79	26.193277890088673	23.833893982575212	23.752870129972123	26.21995799736399
80-84	26.179847815245257	23.850497123440917	23.775553429507216	26.19410163180661
85-89	26.190395860103738	23.92147736753077	23.722702007124873	26.16542476524062
90-94	26.202640899142178	23.852844308062828	23.717814278856427	26.22670051393857
95-99	26.17679524834211	23.842805864222054	23.778403262534	26.201995624901837
100-104	26.189515381418616	23.896758518324166	23.764221213975965	26.149504886281257
105-109	26.186252826560025	23.835691002046524	23.745460020274788	26.232596151118663
110-114	26.16469607412459	23.85234676829267	23.783453244283926	26.199503913298816
115-119	26.210045445346637	23.886689508445365	23.7301750270546	26.173090019153406
120-124	26.201613666960675	23.819692679077107	23.74692725500209	26.231766398960122
125-129	26.178648444014936	23.82681339735199	23.779194769486306	26.215343389146767
130-134	26.209176338905227	23.880269765756402	23.714363110358285	26.19619078498009
135-139	26.207134333880127	23.82409062393876	23.743287790687045	26.225487251494073
140-144	26.183083408540742	23.825975863766484	23.76664211494243	26.224298612750342
145-149	26.220057388211828	23.879939152140768	23.713913518699545	26.18608994094786
150	26.367280213772542	23.453433077597698	23.612985295137314	26.56630141349245
>>END_MODULE
>>Per base GC content	pass
#Base	%GC
1	49.98854045474536
2	46.90584283628795
3	48.915221911354976
4	50.990913258014935
5	51.129703469236134
6	52.30367940113992
7	52.226558742337104
8	50.96574469841077
9	51.491762612236094
10-14	51.35596019226713
15-19	51.6100418373452
20-24	51.801971842686925
25-29	52.01483807272667
30-34	52.21988618434813
35-39	52.24069550282424
40-44	52.28499855161445
45-49	52.39103841067894
50-54	52.317606724522925
55-59	52.32560394862279
60-64	52.41778229266407
65-69	52.35412050613116
70-74	52.38107891752504
75-79	52.41323588745267
80-84	52.37394944705187
85-89	52.35582062534435
90-94	52.42934141308074
95-99	52.37879087324395
100-104	52.339020267699865
105-109	52.41884897767869
110-114	52.36419998742341
115-119	52.38313546450004
120-124	52.4333800659208
125-129	52.3939918331617
130-134	52.40536712388531
135-139	52.43262158537419
140-144	52.407382021291085
145-149	52.406147329159694
150	52.93358162726499
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	0.5
6	0.0
7	0.0
8	0.0
9	0.5
10	2.5
11	7.5
12	20.5
13	41.0
14	79.5
15	107.0
16	140.5
17	299.0
18	490.0
19	670.5
20	1076.0
21	1541.5
22	2024.0
23	2672.0
24	3578.5
25	4816.5
26	6484.0
27	8401.5
28	10132.0
29	12035.5
30	14688.5
31	17632.0
32	20757.0
33	24110.0
34	27900.0
35	32007.0
36	37338.5
37	43706.5
38	50231.5
39	57551.5
40	66148.0
41	76400.5
42	87500.0
43	99573.0
44	113976.5
45	130310.0
46	148020.5
47	170171.0
48	195199.0
49	220824.0
50	245182.0
51	267251.0
52	287227.5
53	301426.5
54	310334.0
55	312714.0
56	305778.0
57	289827.0
58	267967.0
59	242294.0
60	211800.0
61	179546.0
62	147849.5
63	119053.0
64	93770.0
65	70970.5
66	52290.0
67	37668.0
68	26849.0
69	18743.5
70	12595.5
71	8270.0
72	5409.5
73	3623.0
74	2490.0
75	1808.0
76	1175.0
77	770.5
78	760.0
79	709.0
80	671.0
81	569.5
82	484.0
83	479.0
84	311.0
85	177.0
86	66.0
87	21.0
88	3.0
89	0.5
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.643733252491038E-5
2	0.036182271197236014
3	0.005246975883587095
4	0.0
5	3.643733252491038E-5
6	8.744959805978491E-4
7	0.0016761172961458775
8	5.465599878736557E-5
9	0.0
10-14	2.3319892815942646E-4
15-19	0.0
20-24	0.0
25-29	4.190293240364694E-4
30-34	0.0
35-39	6.485845189434048E-4
40-44	1.0931199757473114E-4
45-49	2.550613276743727E-5
50-54	0.03007392264246277
55-59	0.05111160624465542
60-64	2.9245615204912326E-4
65-69	3.660507009504872E-5
70-74	7.330394016375294E-6
75-79	0.0
80-84	0.0
85-89	0.0
90-94	6.999423689556845E-5
95-99	2.2131473345296135E-5
100-104	3.693389980579047E-6
105-109	3.6982940583133692E-6
110-114	8.33248064281422E-4
115-119	5.674007150732411E-4
120-124	1.522675365871965E-4
125-129	5.207039903071696E-4
130-134	6.332595436962682E-5
135-139	0.0
140-144	0.0
145-149	0.001029646364399187
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	7567.0
55-59	7594.0
60-64	7089.0
65-69	7103.0
70-74	6942.0
75-79	7079.0
80-84	6854.0
85-89	6918.0
90-94	6793.0
95-99	7001.0
100-104	7129.0
105-109	7280.0
110-114	7497.0
115-119	7651.0
120-124	7845.0
125-129	8118.0
130-134	8516.0
135-139	8733.0
140-144	8993.0
145-149	96482.0
150-151	5249692.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	62.83804905249107
#Duplication Level	Relative count
1	100.0
2	64.50802528077115
3	31.924930659755375
4	14.113581594143591
5	6.396353385168008
6	3.218069385713636
7	2.055199381621425
8	1.4550084117673805
9	1.1128540899377075
10++	2.5610421497749285
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	2119900	3.4697206	4.197006	7
AAAAA	2137295	3.3878884	4.6052394	1
>>END_MODULE