##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	AVT98_2P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3876439	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.21413544750737	37.0	37.0	37.0	37.0	37.0
2	36.30541613062917	37.0	37.0	37.0	37.0	37.0
3	36.374066765915835	37.0	37.0	37.0	37.0	37.0
4	36.379050205613964	37.0	37.0	37.0	37.0	37.0
5	36.418102542049546	37.0	37.0	37.0	37.0	37.0
6	36.39606814398472	37.0	37.0	37.0	37.0	37.0
7	36.40155281690232	37.0	37.0	37.0	37.0	37.0
8	36.40962568996958	37.0	37.0	37.0	37.0	37.0
9	36.39912584720152	37.0	37.0	37.0	37.0	37.0
10-14	36.4222515561318	37.0	37.0	37.0	37.0	37.0
15-19	36.42437690880728	37.0	37.0	37.0	37.0	37.0
20-24	36.423352411839836	37.0	37.0	37.0	37.0	37.0
25-29	36.38457470890165	37.0	37.0	37.0	37.0	37.0
30-34	36.35089611883484	37.0	37.0	37.0	37.0	37.0
35-39	36.33566007358816	37.0	37.0	37.0	37.0	37.0
40-44	36.32274930677356	37.0	37.0	37.0	37.0	37.0
45-49	36.29271571150739	37.0	37.0	37.0	37.0	37.0
50-54	36.2657196118779	37.0	37.0	37.0	37.0	37.0
55-59	36.21568361113777	37.0	37.0	37.0	37.0	37.0
60-64	36.219237996359894	37.0	37.0	37.0	37.0	37.0
65-69	36.168129741166666	37.0	37.0	37.0	37.0	37.0
70-74	36.170793721168884	37.0	37.0	37.0	37.0	37.0
75-79	36.135025160417115	37.0	37.0	37.0	37.0	37.0
80-84	36.08890898771733	37.0	37.0	37.0	37.0	37.0
85-89	36.055547204061085	37.0	37.0	37.0	37.0	37.0
90-94	36.03139955874608	37.0	37.0	37.0	37.0	37.0
95-99	36.00658602708621	37.0	37.0	37.0	37.0	37.0
100-104	35.95744150149836	37.0	37.0	37.0	37.0	37.0
105-109	35.88733883795616	37.0	37.0	37.0	37.0	37.0
110-114	35.887509802427914	37.0	37.0	37.0	37.0	37.0
115-119	35.839821206489695	37.0	37.0	37.0	37.0	37.0
120-124	35.79677298353629	37.0	37.0	37.0	37.0	37.0
125-129	35.7712589976257	37.0	37.0	37.0	37.0	37.0
130-134	35.661819682041845	37.0	37.0	37.0	37.0	37.0
135-139	35.605643882218	37.0	37.0	37.0	37.0	37.0
140-144	35.52839290591315	37.0	37.0	37.0	37.0	37.0
145-149	35.45939801548168	37.0	37.0	37.0	37.0	37.0
150	36.21527199693817	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	1.0
26	30.0
27	268.0
28	1365.0
29	4930.0
30	13138.0
31	29678.0
32	58043.0
33	109777.0
34	230642.0
35	703618.0
36	2598607.0
37	126342.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.280640377219527	29.745236950564514	21.102317339128295	25.87180533308766
2	28.657280560844633	29.414083389420032	23.78899809851258	18.13963795122276
3	22.37241447627578	26.123202248248973	24.928549114277303	26.575834161197943
4	24.46859088973153	25.10058845244308	23.667881785318947	26.762938872506442
5	25.42771858398907	24.758496135241646	23.99000732373191	25.823777957037375
6	25.694306142728745	23.283892118126463	24.13458095526041	26.88722078388438
7	26.726591080112446	23.87294111941398	23.556000752236784	25.844467048236798
8	24.902726445585756	23.65617000551279	25.07486897123881	26.366234577662645
9	25.786217711667845	23.285752723053296	24.828586235975852	26.099443329303
10-14	25.81939931852888	24.277550530162078	24.13650348830622	25.76654666300282
15-19	25.823756497203824	23.962789745469344	24.19670559313646	26.016748164190375
20-24	26.002310678117937	23.904833453022256	24.034411180285158	26.05844468857465
25-29	26.099465540271765	23.893306174379674	23.868659836685122	26.13856844866344
30-34	26.18311521741071	23.790780421589943	23.776137833089866	26.24996652790948
35-39	26.205170006760348	23.763004303807303	23.759026415090055	26.27279927434229
40-44	26.254826354428438	23.809629945613803	23.676651743637592	26.258891956320163
45-49	26.276075037352598	23.727807393458747	23.677761441472008	26.31835612771665
50-54	26.272248563506224	23.724570931256064	23.6931789315072	26.310001573730514
55-59	26.290861417428324	23.789478366864927	23.656715858178025	26.262944357528724
60-64	26.308828200029026	23.694122922897858	23.63906465933652	26.357984217736597
65-69	26.27548590415052	23.73042410394596	23.691496435333327	26.302593556570198
70-74	26.31533731834909	23.772946147683758	23.634086835693115	26.277629698274037
75-79	26.32761108464527	23.726487599189603	23.64284928348419	26.30305203268094
80-84	26.27085019799174	23.722223874980493	23.69018820045418	26.316737726573585
85-89	26.30102054955468	23.792972619731223	23.63902586349427	26.266980967219826
90-94	26.309740426248577	23.705830908142747	23.624849492379738	26.359579173228937
95-99	26.303543885488917	23.71884936133977	23.67000043406106	26.30760631911025
100-104	26.307834887005814	23.767218290781813	23.626278615108728	26.29866820710365
105-109	26.330967001133487	23.683404482806814	23.620126738886317	26.365501777173385
110-114	26.276675730311393	23.729392637216407	23.660564990895864	26.333366641576333
115-119	26.30797765994563	23.776186351545288	23.58833589865191	26.32750008985717
120-124	26.33691622089484	23.685752391266664	23.609594343527338	26.367737044311156
125-129	26.290008444005903	23.698075705881823	23.646875576878987	26.365040273233287
130-134	26.318853373268837	23.721021520285703	23.607595216804306	26.352529889641158
135-139	26.370336910626698	23.63000529129725	23.571109132043315	26.428548666032736
140-144	26.327485145528613	23.653326342951793	23.596742221922376	26.422446289597218
145-149	26.372946103046687	23.66478703286779	23.568402455498013	26.393864408587515
150	26.800678071060712	22.883230637827616	23.34823018061049	26.96786111050118
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	49.15244571030719
2	46.796918512067386
3	48.948248637473725
4	51.23152976223797
5	51.25149654102644
6	52.58152692661312
7	52.571058128349236
8	51.268961023248394
9	51.88566104097085
10-14	51.5859459815317
15-19	51.84050466139419
20-24	52.06075536669259
25-29	52.23803398893521
30-34	52.43308174532019
35-39	52.477969281102645
40-44	52.5137183107486
45-49	52.594431165069246
50-54	52.58225013723674
55-59	52.553805774957056
60-64	52.66681241776563
65-69	52.578079460720716
70-74	52.59296701662313
75-79	52.63066311732621
80-84	52.58758792456533
85-89	52.56800151677451
90-94	52.66931959947752
95-99	52.61115020459917
100-104	52.606503094109456
105-109	52.69646877830687
110-114	52.61004237188773
115-119	52.6354777498028
120-124	52.70465326520599
125-129	52.6550487172392
130-134	52.67138326291
135-139	52.79888557665944
140-144	52.74993143512583
145-149	52.7668105116342
150	53.76853918156189
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	11.0
2	1.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	4.5
10	7.0
11	11.0
12	17.5
13	36.0
14	65.0
15	78.5
16	112.0
17	217.0
18	320.5
19	452.5
20	778.0
21	1099.5
22	1384.0
23	1804.0
24	2504.0
25	3398.0
26	4397.0
27	5629.0
28	6834.0
29	8127.0
30	9860.5
31	11896.5
32	14087.0
33	16432.0
34	19085.5
35	21904.0
36	25347.0
37	29383.5
38	33530.5
39	38372.0
40	44366.5
41	51355.5
42	58630.0
43	66723.5
44	76666.0
45	88595.5
46	102154.0
47	117382.0
48	134201.0
49	152151.5
50	170160.0
51	187048.0
52	201624.0
53	213346.0
54	221169.0
55	224191.0
56	220958.5
57	210142.5
58	195115.5
59	176966.0
60	155383.0
61	132339.0
62	109506.0
63	88187.5
64	68884.5
65	52226.0
66	38624.5
67	27863.5
68	19858.0
69	13703.0
70	8967.5
71	5870.5
72	3825.0
73	2473.5
74	1700.0
75	1161.5
76	728.0
77	476.5
78	414.0
79	372.5
80	300.5
81	228.0
82	206.0
83	199.0
84	132.0
85	80.5
86	30.0
87	8.5
88	2.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.5
99	16.5
100	72.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034774183212995226
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0013156404628061993
7	0.0
8	0.0
9	0.0
10-14	4.6434369275512915E-5
15-19	5.675311800340467E-5
20-24	3.095624618367527E-5
25-29	2.5796871819729397E-5
30-34	0.0019399247608436507
35-39	2.7860621565307746E-4
40-44	3.663155798401574E-4
45-49	4.1274994911567036E-5
50-54	3.0978937523640807E-5
55-59	1.1897194993163769E-4
60-64	1.3473062813232518E-4
65-69	1.713042890544969E-4
70-74	4.6802981911583546E-5
75-79	2.604905130136632E-5
80-84	2.5052273133660454E-4
85-89	6.588262852864221E-4
90-94	1.047692700415887E-5
95-99	6.823204269100459E-5
100-104	5.259139082087746E-6
105-109	6.324081342863864E-5
110-114	2.640716242522746E-5
115-119	1.6939172964995254E-4
120-124	5.305701804278178E-6
125-129	1.5423303739736324E-4
130-134	2.1327744895430864E-5
135-139	2.9942578689096794E-4
140-144	5.362915173429973E-6
145-149	1.614479426446497E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	7187.0
55-59	6899.0
60-64	6859.0
65-69	6706.0
70-74	7170.0
75-79	6878.0
80-84	6895.0
85-89	7082.0
90-94	7276.0
95-99	7505.0
100-104	7708.0
105-109	8056.0
110-114	8525.0
115-119	8645.0
120-124	8711.0
125-129	9448.0
130-134	10037.0
135-139	11017.0
140-144	11493.0
145-149	124489.0
150-151	3597853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	57.393145615984096
#Duplication Level	Relative count
1	100.0
2	55.0055382926375
3	24.608643657429646
4	9.890508444670987
5	3.812109746414813
6	1.4948488977327308
7	0.6175441348010625
8	0.2764245174823804
9	0.1617377495907545
10++	0.1784016389425292
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1434755	3.428374	4.170842	7
AAAAA	1463575	3.3778899	4.984255	1
>>END_MODULE