##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	AVT98_1P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	3876439	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.43446678768839	37.0	37.0	37.0	37.0	37.0
2	36.46927734449065	37.0	37.0	37.0	37.0	37.0
3	36.53849860658197	37.0	37.0	37.0	37.0	37.0
4	36.55711827272401	37.0	37.0	37.0	37.0	37.0
5	36.58246679491152	37.0	37.0	37.0	37.0	37.0
6	36.59503064539388	37.0	37.0	37.0	37.0	37.0
7	36.586064942592934	37.0	37.0	37.0	37.0	37.0
8	36.59072488951845	37.0	37.0	37.0	37.0	37.0
9	36.59624645196274	37.0	37.0	37.0	37.0	37.0
10-14	36.59388500631636	37.0	37.0	37.0	37.0	37.0
15-19	36.58387984436231	37.0	37.0	37.0	37.0	37.0
20-24	36.567018183441036	37.0	37.0	37.0	37.0	37.0
25-29	36.53864626787627	37.0	37.0	37.0	37.0	37.0
30-34	36.52463464535363	37.0	37.0	37.0	37.0	37.0
35-39	36.51415002273994	37.0	37.0	37.0	37.0	37.0
40-44	36.49934411453398	37.0	37.0	37.0	37.0	37.0
45-49	36.4825263082948	37.0	37.0	37.0	37.0	37.0
50-54	36.464049735075506	37.0	37.0	37.0	37.0	37.0
55-59	36.45985941770739	37.0	37.0	37.0	37.0	37.0
60-64	36.452579753604994	37.0	37.0	37.0	37.0	37.0
65-69	36.4402252843548	37.0	37.0	37.0	37.0	37.0
70-74	36.4362380335734	37.0	37.0	37.0	37.0	37.0
75-79	36.42457828156305	37.0	37.0	37.0	37.0	37.0
80-84	36.40604812754974	37.0	37.0	37.0	37.0	37.0
85-89	36.38691826565427	37.0	37.0	37.0	37.0	37.0
90-94	36.39774365017916	37.0	37.0	37.0	37.0	37.0
95-99	36.36916592783871	37.0	37.0	37.0	37.0	37.0
100-104	36.358193057270704	37.0	37.0	37.0	37.0	37.0
105-109	36.34601010129262	37.0	37.0	37.0	37.0	37.0
110-114	36.3318432820199	37.0	37.0	37.0	37.0	37.0
115-119	36.312140594034	37.0	37.0	37.0	37.0	37.0
120-124	36.27538135567929	37.0	37.0	37.0	37.0	37.0
125-129	36.280917209416764	37.0	37.0	37.0	37.0	37.0
130-134	36.22495421472902	37.0	37.0	37.0	37.0	37.0
135-139	36.21715091580058	37.0	37.0	37.0	37.0	37.0
140-144	36.17827963019407	37.0	37.0	37.0	37.0	37.0
145-149	36.1163568685111	37.0	37.0	37.0	37.0	37.0
150	36.52042873870941	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
26	13.0
27	107.0
28	701.0
29	3402.0
30	10599.0
31	25730.0
32	49230.0
33	83256.0
34	139007.0
35	306664.0
36	2771743.0
37	485987.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	23.617841946653087	29.497569676078918	21.1958504173161	25.688737959951897
2	28.804514063211855	28.9176753916701	24.02811997062555	18.249690574492497
3	22.39132358331964	25.839462455103767	25.224722999639614	26.544490961936972
4	24.44751484545481	25.044738224953367	23.862622370686086	26.645124558905735
5	25.498376215903306	24.643080930720178	24.009483961955805	25.849058891420707
6	25.722041730062493	23.230966160170755	24.220070074640688	26.82692203512606
7	26.629900446390447	23.839437717787227	23.61847405824721	25.91218777757512
8	24.998303855677854	23.639479429445426	24.99546619977768	26.366750515099035
9	25.801953803477883	23.346272184342382	24.706154282319417	26.14561972986032
10-14	25.86573547355305	24.253435155621958	24.0638817517791	25.816947619045898
15-19	25.87488671948662	23.97263055087414	24.14049079580512	26.01199193383412
20-24	26.026376269560803	23.921367007193975	23.985498030537823	26.066758692707403
25-29	26.128128640147175	23.86864412922894	23.87904040374074	26.124186826883143
30-34	26.213532574612934	23.76960916965287	23.764578779648023	26.252279476086173
35-39	26.244743968352523	23.749044088185244	23.764651338163034	26.241560605299203
40-44	26.27971186957927	23.767700201138208	23.700906940622566	26.251680988659952
45-49	26.30680374436435	23.703300890327437	23.694044972718515	26.295850392589692
50-54	26.25735335493673	23.735765014782856	23.74625007937364	26.260631550906773
55-59	26.320945865771822	23.755333715347362	23.676750778862427	26.246969640018385
60-64	26.299065498958495	23.730214197719484	23.661148392733914	26.309571910588108
65-69	26.291373309645934	23.70107096336712	23.73323855053257	26.27431717645438
70-74	26.297594908080015	23.767282305593618	23.66503675163673	26.270086034689637
75-79	26.312700044283098	23.71323274161787	23.67229882848711	26.30176838561192
80-84	26.276496720546028	23.73158044444926	23.71749878477985	26.274424050224866
85-89	26.285159340792003	23.795505910123936	23.675132506956682	26.244202242127372
90-94	26.292755524494382	23.724474862252208	23.662728508898155	26.32004110435525
95-99	26.273982300588784	23.711488082236432	23.72382400698551	26.290705610189274
100-104	26.284011867079325	23.773290735065192	23.68770076594775	26.25499663190773
105-109	26.304765511444717	23.722483496560827	23.64598281768219	26.32676817431227
110-114	26.28339683082125	23.720473195525386	23.690783739672096	26.30534623398127
115-119	26.305163039420027	23.757424292045886	23.673623946369464	26.263788722164623
120-124	26.306276522378745	23.70814187790132	23.66150061738979	26.324080982330138
125-129	26.28325332223364	23.722664310021486	23.714821601319727	26.27926076642515
130-134	26.299219736007196	23.759273122824546	23.67425695105194	26.26725019011632
135-139	26.308869190864126	23.711629595294486	23.66124512399808	26.318256089843313
140-144	26.30268290315832	23.69603704280273	23.683013884319475	26.31826616971948
145-149	26.331703046630466	23.729011207032926	23.654854163921954	26.28443158241465
150	26.506019218092796	23.34986398116521	23.45019777932179	26.6939190214202
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	49.30657990660498
2	47.05420463770436
3	48.93581454525661
4	51.092639404360554
5	51.34743510732401
6	52.54896376518856
7	52.542088223965564
8	51.36505437077689
9	51.9475735333382
10-14	51.68268309259895
15-19	51.88687865332074
20-24	52.09313496226821
25-29	52.25231546703032
30-34	52.465812050699114
35-39	52.48630457365172
40-44	52.53139285823922
45-49	52.602654136954044
50-54	52.5179849058435
55-59	52.56791550579021
60-64	52.60863740954661
65-69	52.565690486100316
70-74	52.56768094276965
75-79	52.61446842989502
80-84	52.550920770770894
85-89	52.52936158291938
90-94	52.61279662884964
95-99	52.56468791077806
100-104	52.53900849898706
105-109	52.631533685756985
110-114	52.588743064802514
115-119	52.56895176158465
120-124	52.63035750470889
125-129	52.56251408865879
130-134	52.56646992612352
135-139	52.62712528070743
140-144	52.6209490728778
145-149	52.61613462904512
150	53.199938239513
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	0.5
3	0.0
4	0.0
5	0.0
6	0.5
7	1.5
8	2.0
9	2.0
10	2.0
11	4.5
12	15.5
13	37.5
14	58.0
15	75.5
16	107.0
17	207.0
18	329.0
19	425.0
20	703.0
21	1032.5
22	1329.5
23	1777.0
24	2455.5
25	3377.5
26	4397.5
27	5485.0
28	6778.0
29	8156.0
30	9750.5
31	11746.0
32	13979.0
33	16288.0
34	18949.5
35	21955.5
36	25253.5
37	28961.5
38	33318.0
39	38635.0
40	44382.0
41	50865.0
42	58334.0
43	66644.0
44	76595.0
45	88629.5
46	102162.5
47	117276.0
48	134774.0
49	153244.5
50	171124.5
51	187464.0
52	201779.0
53	212926.0
54	220395.5
55	223743.0
56	220570.5
57	209848.0
58	195052.5
59	177250.0
60	155067.5
61	132324.0
62	109326.0
63	87518.5
64	68566.5
65	51878.5
66	38165.5
67	27321.0
68	19519.5
69	13636.5
70	9016.0
71	5718.5
72	3710.0
73	2508.0
74	1677.0
75	1129.5
76	698.0
77	436.0
78	399.0
79	360.0
80	332.0
81	271.0
82	249.0
83	246.5
84	134.5
85	83.5
86	37.5
87	7.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	2.5796871819729397E-5
2	0.05995193010905112
3	0.0
4	0.0
5	0.0
6	2.5796871819729397E-5
7	0.006088061749456137
8	0.0
9	0.0
10-14	0.0016613185451905732
15-19	0.0
20-24	0.0
25-29	0.0013001623397143616
30-34	0.0
35-39	9.132092624184207E-4
40-44	0.0
45-49	0.0
50-54	0.0
55-59	1.240863303758218E-4
60-64	0.0013411158788327175
65-69	9.075087324379558E-4
70-74	0.0
75-79	3.536394675791397E-4
80-84	3.228772487358835E-4
85-89	5.214807299311792E-6
90-94	2.819937143601069E-4
95-99	1.5687905231665388E-5
100-104	1.0996557815580918E-4
105-109	3.6706049461087024E-5
110-114	5.25105311182921E-6
115-119	0.0019824362458757305
120-124	1.8431083991555613E-4
125-129	5.274181767396243E-6
130-134	1.109318768928742E-4
135-139	1.058274857474188E-5
140-144	2.6502146991932113E-5
145-149	0.0016939273924308313
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	5847.0
55-59	5805.0
60-64	5783.0
65-69	5631.0
70-74	5493.0
75-79	5349.0
80-84	5165.0
85-89	5354.0
90-94	5316.0
95-99	5203.0
100-104	5297.0
105-109	5283.0
110-114	5359.0
115-119	5363.0
120-124	5814.0
125-129	5807.0
130-134	6233.0
135-139	6411.0
140-144	6351.0
145-149	64941.0
150-151	3704634.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	58.048776422645666
#Duplication Level	Relative count
1	100.0
2	56.27234803337306
3	25.4787445371474
4	10.154946364719905
5	3.8528009535160908
6	1.5991259435836314
7	0.6624950337703616
8	0.27910210568136673
9	0.16090584028605484
10++	0.18871672626142233
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1455285	3.436012	4.102765	7
AAAAA	1466685	3.3838503	4.736798	1
>>END_MODULE