##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	AVT100_2P.fq	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	4125584	
Filtered Sequences	0	
Sequence length	50-150	
%GC	52	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.152085377488376	37.0	37.0	37.0	37.0	37.0
2	36.26348075811812	37.0	37.0	37.0	37.0	37.0
3	36.34196661612029	37.0	37.0	37.0	37.0	37.0
4	36.33034789741283	37.0	37.0	37.0	37.0	37.0
5	36.349208742325935	37.0	37.0	37.0	37.0	37.0
6	36.32773881225058	37.0	37.0	37.0	37.0	37.0
7	36.35969404573995	37.0	37.0	37.0	37.0	37.0
8	36.336974837986574	37.0	37.0	37.0	37.0	37.0
9	36.324656581953	37.0	37.0	37.0	37.0	37.0
10-14	36.34655801457442	37.0	37.0	37.0	37.0	37.0
15-19	36.33886673983611	37.0	37.0	37.0	37.0	37.0
20-24	36.3281109292648	37.0	37.0	37.0	37.0	37.0
25-29	36.28934705971324	37.0	37.0	37.0	37.0	37.0
30-34	36.26785890191546	37.0	37.0	37.0	37.0	37.0
35-39	36.22954112678351	37.0	37.0	37.0	37.0	37.0
40-44	36.21020980302425	37.0	37.0	37.0	37.0	37.0
45-49	36.17769479424004	37.0	37.0	37.0	37.0	37.0
50-54	36.13010722089866	37.0	37.0	37.0	37.0	37.0
55-59	36.11053992079464	37.0	37.0	37.0	37.0	37.0
60-64	36.06149656339591	37.0	37.0	37.0	37.0	37.0
65-69	36.04730919333272	37.0	37.0	37.0	37.0	37.0
70-74	36.01721016629225	37.0	37.0	37.0	37.0	37.0
75-79	35.990394756041965	37.0	37.0	37.0	37.0	37.0
80-84	35.96713018080476	37.0	37.0	37.0	37.0	37.0
85-89	35.921258105608146	37.0	37.0	37.0	37.0	37.0
90-94	35.90050122052977	37.0	37.0	37.0	37.0	37.0
95-99	35.856822194622985	37.0	37.0	37.0	37.0	37.0
100-104	35.80526157397861	37.0	37.0	37.0	37.0	37.0
105-109	35.76569899960207	37.0	37.0	37.0	37.0	37.0
110-114	35.713095006793914	37.0	37.0	37.0	37.0	37.0
115-119	35.719216490383815	37.0	37.0	37.0	37.0	37.0
120-124	35.658744473136835	37.0	37.0	37.0	37.0	37.0
125-129	35.60065153437907	37.0	37.0	37.0	37.0	37.0
130-134	35.537797680684335	37.0	37.0	37.0	37.0	37.0
135-139	35.511312563592426	37.0	37.0	37.0	37.0	37.0
140-144	35.38025266805364	37.0	37.0	37.0	37.0	37.0
145-149	35.364858874021174	37.0	37.0	37.0	32.2	37.0
150	36.16669557312066	37.0	37.0	37.0	37.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	3.0
26	14.0
27	158.0
28	849.0
29	3063.0
30	9404.0
31	24099.0
32	55745.0
33	125895.0
34	311410.0
35	996950.0
36	2532419.0
37	65575.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.992881439073507	29.993127191117214	21.28768248544835	25.726308884360925
2	28.366141617768537	29.338125220574835	24.11537372648333	18.180359435173298
3	22.4682861502492	26.212055020614063	24.858213163423482	26.461445665713256
4	24.467808678722818	25.094435115125517	23.642786087981726	26.794970118169935
5	25.35713731680169	24.787278601041695	24.01228529100365	25.843298791152964
6	25.636654506671835	23.306104640593375	24.161287585897636	26.895953266837147
7	26.802993224716793	23.932296615460988	23.594138429856233	25.670571729965985
8	24.82421882574685	23.67407862741372	25.111765994826428	26.389936552012998
9	25.692968559117936	23.357081082338887	24.865085767251376	26.0848645912918
10-14	25.795017355876993	24.313144494759836	24.174560229956704	25.717277919406467
15-19	25.78829085416715	24.00702708923703	24.26980121101813	25.93488084557769
20-24	25.929623539358303	23.967646762252325	24.134357705478788	25.968371992910576
25-29	26.02100938921617	23.938443623981478	23.990150242971662	26.05039674383069
30-34	26.099820049719018	23.873536449627494	23.887633847717073	26.13900965293641
35-39	26.11439253206334	23.844483593110695	23.907504973841277	26.133618900984683
40-44	26.165905028874654	23.90981273857084	23.835454822184566	26.088827410369937
45-49	26.181907822019863	23.83324639614658	23.78502049649213	26.199825285341422
50-54	26.16570560279018	23.82740869286824	23.821616842932453	26.185268861409135
55-59	26.19604163762803	23.874051903179915	23.78617930760942	26.14372715158263
60-64	26.20808923588967	23.80181017355667	23.75321086092456	26.236889729629098
65-69	26.165142531222973	23.81981991290158	23.84786272492794	26.16717483094751
70-74	26.21232607339764	23.857216252664042	23.76469493546355	26.16576273847477
75-79	26.21528627496347	23.792578468849527	23.76811146354008	26.22402379264692
80-84	26.18652883443528	23.814524244773224	23.814940578413797	26.184006342377703
85-89	26.191896630928813	23.870357635968922	23.763837920841937	26.173907812260328
90-94	26.19643643034279	23.81498510972322	23.752733169376352	26.235845290557634
95-99	26.188320908331114	23.81803236624018	23.78618719307897	26.20745953234974
100-104	26.22537607907191	23.844019397867847	23.76237824085513	26.168226282205115
105-109	26.220780011958677	23.786998271075092	23.720667763373257	26.271553953592974
110-114	26.22877865944464	23.777980100110906	23.756653339753825	26.236587900690633
115-119	26.217230585020744	23.842282555028607	23.709309861638964	26.23117699831168
120-124	26.23969448104557	23.753474341060198	23.72091670822466	26.28591446966957
125-129	26.193408189417468	23.77645723175982	23.764686925835694	26.265447652987024
130-134	26.260066793764164	23.79614696950751	23.692329209021793	26.251457027706525
135-139	26.281113274488398	23.73160794623472	23.694739499119255	26.29253928015763
140-144	26.24362239277908	23.71633226899933	23.711916077660714	26.32812926056088
145-149	26.28104750985431	23.74655319659036	23.67449774067384	26.29790155288149
150	26.796702044323812	22.85935481842642	23.276917716163073	27.067025421086687
>>END_MODULE
>>Per base GC content	warn
#Base	%GC
1	48.71919032343443
2	46.546501052941835
3	48.929731815962455
4	51.262778796892746
5	51.20043610795466
6	52.532607773508985
7	52.473564954682786
8	51.21415537775985
9	51.777833150409734
10-14	51.512295275283456
15-19	51.72317169974484
20-24	51.89799553226888
25-29	52.071406133046864
30-34	52.23882970265543
35-39	52.24801143304803
40-44	52.25473243924459
45-49	52.381733107361285
50-54	52.350974464199304
55-59	52.33976878921066
60-64	52.44497896551877
65-69	52.33231736217048
70-74	52.378088811872416
75-79	52.43931006761039
80-84	52.37053517681298
85-89	52.36580444318915
90-94	52.43228172090042
95-99	52.39578044068085
100-104	52.393602361277026
105-109	52.49233396555165
110-114	52.46536656013527
115-119	52.448407583332425
120-124	52.52560895071514
125-129	52.45885584240448
130-134	52.511523821470696
135-139	52.573652554646024
140-144	52.57175165333996
145-149	52.5789490627358
150	53.863727465410506
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26.0
1	14.5
2	3.0
3	1.5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	2.5
11	7.0
12	18.0
13	47.5
14	72.5
15	79.0
16	108.0
17	206.0
18	352.5
19	511.5
20	855.0
21	1185.5
22	1520.5
23	2027.5
24	2769.0
25	3699.0
26	4810.5
27	6220.5
28	7641.0
29	9318.0
30	11206.5
31	13547.0
32	16149.0
33	18564.5
34	21330.0
35	24351.0
36	28189.5
37	32807.5
38	37992.0
39	43679.0
40	50217.5
41	57439.0
42	65326.0
43	74569.5
44	85301.5
45	97604.5
46	110994.0
47	126894.0
48	144884.0
49	164309.5
50	183005.0
51	199541.0
52	214107.5
53	225129.5
54	232460.5
55	234245.0
56	229715.5
57	218289.5
58	201934.0
59	182869.5
60	160091.0
61	136315.5
62	113264.5
63	91318.5
64	71926.5
65	54837.0
66	40287.5
67	29136.5
68	20851.5
69	14628.5
70	9926.5
71	6515.5
72	4255.0
73	2802.0
74	1967.0
75	1395.5
76	891.0
77	556.0
78	483.0
79	446.5
80	407.0
81	329.0
82	297.5
83	281.5
84	174.5
85	109.5
86	49.5
87	22.0
88	2.5
89	1.0
90	0.5
91	0.0
92	0.5
93	1.5
94	1.0
95	0.0
96	0.5
97	0.5
98	1.5
99	16.0
100	58.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08587875074171317
2	0.0
3	0.017694464589740506
4	0.0
5	0.0
6	8.241257480153112E-4
7	0.0
8	0.0
9	0.0
10-14	1.7936854515627365E-4
15-19	0.001541599928640406
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0032043948202242394
45-49	0.0
50-54	0.0
55-59	4.858328705775241E-6
60-64	0.0
65-69	0.0016277627312611003
70-74	0.004208000179645483
75-79	0.0
80-84	1.3224698152387217E-4
85-89	0.0017517446174211836
90-94	0.0
95-99	0.0
100-104	0.0
105-109	1.236216372874901E-4
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	9.147088107885912E-4
150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
50-54	6351.0
55-59	6367.0
60-64	6471.0
65-69	6663.0
70-74	6772.0
75-79	6745.0
80-84	7391.0
85-89	7153.0
90-94	7571.0
95-99	7861.0
100-104	8425.0
105-109	8141.0
110-114	8928.0
115-119	9848.0
120-124	9981.0
125-129	10921.0
130-134	11369.0
135-139	11888.0
140-144	12644.0
145-149	147184.0
150-151	3816910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	55.221795319594825
#Duplication Level	Relative count
1	100.0
2	52.17927235607943
3	22.454305579630592
4	9.16681111688912
5	3.9473430789083417
6	1.9751160416787041
7	1.1247857321700276
8	0.7155101018855569
9	0.4054236243523815
10++	0.6317289728626182
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	1575685	3.4569557	4.1291795	8
AAAAA	1597795	3.4152596	5.107655	1
>>END_MODULE